Genetic Engineering Technology Technology Offers

Centre Technology Transfer CITTRU posted this:

The method of the invention is a solution that allows the simultaneous isolation of microbial DNA from the blood. In this method the isolation is carried out by compilation of enzymatic, mechanical and thermal lysis. This approach enables to obtain DNA from all types of organisms, irrespective of the structure of the cells.The obtained precipitate is subjected to further preparations, using a commercial DNA isolation kit according to the protocol provided by the manufacturer's procedure. The procedure results in obtaining the DNA ready for further stages of the analysis, e.g. performing a PCR reaction to detect fungi. The most important and most difficult problem in the treatment of bloodstream infections, determining the effectiveness of therapy and, consequently, the cost and time of hospitalization, is the effective diagnosis of factors responsible for the systemic inflammatory response in the course of sepsis. Determination of etiologic factors allows for selection of appropriate antibiotic therapy. The material subjected to diagnostic testing is blood taken from the patient showing clinical signs of sepsis. Currently, the "gold standard" diagnostic method is testing for microbial growth after inoculation in culture media specific to selected pathogen groups. This method is relatively simple and inexpensive, but also time-consuming – results can be available as late as in 5 days. Moreover, identification of pathogens with this method often fails due to low sensitivity; microbial growth can be detected in only about 15-20% of the cultures.