Cracow University of Technology posted this:The new lupeol derivative and the method of obtaining.This invention relates to a biologically active compound, the derivative of natural triterpene lupeol. This derivative is active for human skin cells, improving their proliferation process. In addition, the substance has the ability to neutralize free radicals. The described derivative can be used as an active ingredient in pharmaceutical and cosmetic preparations dedicated for damaged or burned skin, as well as in anti-aging cosmetics.
University of Huelva posted this:New procedure for the genetic transformation of plant cells and kit for carrying out said methodThe strategy for transformation is extremely simple and does not involves subcloning the gene of interest in any vector. The kit is not based on specific promoters or regulatory DNA sequences, thus it can be applied to many different plant species, from unicellular microalgae to higher plant protoplasts. Since the strategy is not based on specific promoter or regulatory DNA sequences, only the marker and the gene of interest sequences are inserted in the microalgal genome, avoiding the introduction of undeserible DNA sequences in the transformant strain. This is very important given the concern that transfer of exogenous DNA through transgenesis causes in the public opinion
University of Vigo posted this:Method for the sequential immunochemical evaluation on a same biological sample of NCAM (Neural Cell Adhesion Molecule) protein and its PolySialic Acid (PSA) tail, by western blot coupled to chemiluminiscence detection.The method optimizes the expression assays of PSA-NCAM isoforms in biological samples available in small quantities or restricted (as is the case of human specimens, for example). It improves quantitative and qualitative performance of PSA-NCAM analysis by western blot.
RAMOT at Tel Aviv University Ltd. posted this:A novel behavioral bioassay for preclinical testing and characterization of CNS agentsOur team, including zoologists, statisticians, and computer scientists have developed a new hi-res bioassay for preclinical testing and characterization of CNS agents, extracting key animal-centered parameters of behavior via computerized tracking and measurement of free exploration. The features that distinguish our system and method from other existing systems and methods are: elaborate data preparation for analysis and a methodology that secures replicability of results across laboratories, and exposes active management of kinematic variables. Our measured parameters are carefully designed, animal-centered building blocks of behavior. They are selected on the basis of an in-depth understanding of the mouse’s functional world. These quite unexpected and unusual parameters reflect the animal’s cognitive and emotional state and its information processing capacity; therefore they are most prone to be affected by drugs that act on respective CNS structures that mediate these states. Unlike all other existing systems we expose and quantify the rich and highly organized developmental dynamics of self regulated behavioral growth. The dynamics of this process before and after genetic manipulation and/or pharmacological treatment can now be readily measured by our system, articulating the effects of potential CNS drugs on behavioral growth and decay processes. Project ID : 2-2009-11
RAMOT at Tel Aviv University Ltd. posted this:Oral Mucosa-derived Stem Cells: A Unique Population for the Prevention and Treatment of Ischemic Heart FailureStem cells (SC) are considered crucial building blocks for any regenerative strategy. Adult oral mucosa (OM) is a unique source for generating adult pluripotent stem cell (OPSC) populations. This uniqueness consists in the fact that 95% of the OM whole populations in culture (1st – 8th passages) express markers of embryonic and mesenchymal SC. Thus, in contradistinction to known sources which comprise very low numbers of SC, OPSC generation for clinical use is simple and cost effective and does not require cumbersome cost-consuming purification steps. The general objective is to lay the foundation for the development of a variety of products based on the pluripotency and simple and cost effective generation of billions of OPSC. The major aim of the proposed project is to develop a product for the treatment of ischemic heart failure (IHF). This product which will serve as a proof of concept was selected based on medicals needs, market cap and time to market. The specific aims of the proposal are: 1. To develop a cardiac product aimed at treating ischemic heart failure (IHF). The aim is to test the safety and therapeutic effect of OPSC on experimental IHF in a minipig model. 2. To strengthen the intellectual property by fully characterizing the profile of OPSC. The prevalence of IHF continuously increases (1 million new cases every year in the western world) and currently the market cap is estimated in the range of billions. Project ID : 10-2011-260
RAMOT at Tel Aviv University Ltd. posted this:Immunotherapy for Stroke and Cognition in a Cerebral Amyloid Angiopathy Animal ModelCerebral amyloid angiopathy (CAA) is due to amyloid accumulation in the vessel walls leading to hemorrhagic stroke, and cognitive impairment. There are no available treatments to specifically reduce the risk of CAA. In this research we aim to assess brain tissue damage and cognitive impairment resulting from CAA in animal model and to investigate a novel approach to immune therapy. Methods: We have shown that nasal vaccination with a proteosome adjuvant (Protollin) that is well tolerated in humans, decreases amyloid plaques in an Alzheimer’s disease mouse model. It was recently reported that an overexpression of TGF-?1 under the control of an astrocyte promoter GFAP in mice results in CAA. TGF-?1 mice were nasally treated with Protollin on a weekly basis starting at the age of 13 months for three months. Following treatment animals were subjected for MRI and cognition analysis. Results: Here we show that nasal Protollin activates perivascular macrophage and potently decreases vascular amyloid in TGF-?1 mice. Using MRI we found that while PBS treated animals showed a significant enlargement of the lateral ventricles area, Protollin prevents further brain damage and prevents pathological changes in the blood-brain barrier. Vascular risk factors have been found to be associated with vascular dementia. Using an object recognition test and Y-maze, we found significant improvement in cognition with the Protollin treated group. Interpretation :Our study demonstrates that activation of macrophages by Protollin is a novel approach to reduce microhemorrhage, prevent stroke and improve cognition in a model of cerebral amyloid angiopathy. Project ID : 10-2011-259
UATEC - Unidade de Transferência de Tecnologia posted this:Fluorescent compounds, methods of manufacturing and uses thereofA group of researchers from a Portuguese university developed a family of new fluorophores to be used in biological imaging, either in cells or in entire organisms, in fluorescence microscopy techniques, including live cell imaging. The fluorophores are not toxic to cells and organisms, and light up cells by binding to organelles such as the Golgi apparatus, nucleus, and particularly lipid droplets. Importantly, the fluorophores change color with the polarity of the environment. The university is looking for companies that would be interested in developing this technology further through partnership, or in commercializing the probes through licensing or acquisition of the patent.
IMIM Institut Recerca Hospital del Mar posted this:A TREATMENT FOR TRIPLE NEGATIVE BREAST CANCERTriple negative breast cancers are tipically associated with por prognosis, due to aggressive tumor phenotype(s), only partial response to chemoterapy and present lack of clinically established targeted therapies and respresent the focus of increasing interest at the clinical, biological and epidemiological level, as disclosed in Podo et al., Triple –negative breast cancer: Present challenges and new perspectives, Mol. Oncol., 2010, 4, 209-229. There is, thus, a particular need for new active agents suitable for breast cancer treatment, and in particular need for triple negative breast cancers.
Centre Technology Transfer CITTRU posted this:Chitinase A – potential applications in various fields.Methods of chitinase purification, used so far, consisted of multi-stage and expensive procedures. Affinity chromatography was also used with so-called affinity tags. These tags could interfere with the functions of chitinase, and their cut-off was associated with the next step of digestion and purification. The proposed method of Jagiellonian University’s chitinase A purification using affinity chromatography with the involvement of G proteins is fast and efficient single-step. In addition, use of direct conjuction of chitinase and protein G eliminates the need of chitinase tagging, which may disturb protein functions, whereas their cutting requires additional digesting and purifying stage.
Research InstituteView Profile
Germans Trias i Pujol Health Sciences Research Institute (IGTP) posted this:Innovative device for electro-mechanical stimulation for tissue engineering applicationsThe device presented enables the combination of both electrical and mechanical stimulation either independently or simultaneously. The mechanical stimulation is performed with a non-invasive and aseptic novel approach. A sterile, single use device is placed into a standard culture plate. The cells are seeded in a volume contained in the central area of the device, which goes through mechanical stimulation thanks to the embedded magnets and the external magnetic field.
Centre Technology Transfer CITTRU posted this:Anti-histidine and Anti-arginine Tag DNA aptamers for biotechnological applicationsThe subject of the offer are unique sequences of single-stranded DNA aptamers with high affinity to any molecule (peptide, protein or DNA derivative) containing His-Tag and Arg-Tag and their application in biotechnology in analysis of His-Tagged and Arg-Tagged molecules.
Research & Technology Organization
Universidad de Alcalá-OTRI posted this:Semi-automated portable capillary electrophoresis instrument .A research group from the Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering at the University of Alcalá has developed a new portable capillary electrophoresis system (P-CE). The P-CE has a battery system which gives it electrical autonomy. It is designed inside a 35x15x45 cm suitcase. It is a capillary electrophoresis assisted by external pressure. Electrical consumption is not required to generate the pressure, which increases the instrument autonomy. The group is looking for companies working in medical or biological sector or companies which make food, water or forensic analysis to reach licensing agreements, partnerships and commercial agreements with technical assistance.
Technology Transfer OfficeView Profile
Yissum - Research Development Company of the Hebrew University posted this:Research & Services | Modern cell and molecular biology research of diabetesResearch & Services | Modern cell and molecular biology research of diabetes Project ID : 34-2010-2440