Simple, Cost-Effective Bio-Sensor for DNA Detection
Provisional filed Nov. 2015. Project ID : 9-2015-4246
Summary of the technology
Provisional filed Nov. 2015.
Project ID : 9-2015-4246
Description of the technology
Self-assembled peptide-based conjugates provide highly sensitive biosensors
Nano-medicine / biotechnology, Diagnostics, Nanoprocesses, Diagnostics, Homeland Security, Research Tools
Proof of concept
Provisional patent filed
Self-assembled nanostructures generated by a a (modified) peptide-based compound provide a highly sensitive optical sensing platform for DNA detection.
DNA detection by a ligand peptide material conjugate (LM). Schematic representation of the experimental design for detecting target HIV DNA using LM as a sensing platform. In the first step, the fluorescein-labeled probe DNA interacts and adsorbs on the self-assembled aggregated spherical structures of LM; this triggers the florescence quenching. The presence of the target DNA induces fluorescence recovery owing to the conformational change of the dye-labeled SS DN) into a duplex form.
Perform in vitro studies using biological samples from infected animals and in vivo studies.