Food Microbiology / Toxicology / Quality Control Technology Offers

Centre Technology Transfer CITTRU posted this:

Staphylococci (Staphylococcus) are one of the most important human bacterial pathogens. Many methods have been developed for the identification and typing of bacterial isolates of the genus Staphylococcus (Staphylococcus aureus) in recent years. These methods can be divided into two groups: phenotypic and genotypic methods. The results obtained using commercial tests, which are based on biochemical and immunological reactions profiles, are affected by mistakes due to variable expression of phenotypic features and the ambiguity from the interpreting the test results. The overall accuracy of conventional tests is low and it is in the range 50-70%. Among genotypic methods, DNA-DNA hybridisation and 16S rRNA gene sequence analysis have defined the species in the genus Staphylococcus and they are recommended to confirm the results of the new methods that are introduced into general use. However, there is still no universal method for staphylococcal species identification and typing at the same time, which would be widely used in all laboratories and allow to obtain high reproducibility of the results. The proposed method may determine the species identification and phylogenetic relationships in the genus Staphylococcus. It shows a high sensitivity and specificity. The results of this method can be interpret very easy, even in the case of the intraspecific polymorphisms. There is the possibility to differentiate between even very closely related species such as S. delphini, S. intermedius and S. pseudintermedius.