University of Luxembourg

Single cell OMICS technologies

Posted by Lidia from University of LuxembourgResponsive · Innovative Products and Technologies · Luxembourg

Summary of the technology

The Integrative Cell Signalling group at the LCSB investigates changes of cellular heterogeneity in health and disease at single cell resolution. In diverse projects including cell differentiation, cancer heterogeneity and brain chraracterization, we develop and apply single cell RNA sequencing (sc-RNAseq) methods such as Drop-seq, CITE-seq, ATAC-seq, Fluidigm C1, InDrop or SPLiT-seq.

University of Luxembourg

Description of the technology

The recent technology of single cell transcriptomics and epigenomics allows for in depth characterization of cellular states. Investigating tissue heterogeneity at single cell resolution gives new insights into underlying disease mechanisms and is able to identify driving cellular subpopulations . Combining scRNA-seq technology with membrane-associated protein tagging (CITE-seq) can give additional layer of information in understanding mRNA-protein relationship on a temporal scale. In addition, single cell chromatin accessibility technology (ATAC-seq) allows for identification and profiling of distinct regulatory modules across different cell types within heterogeneous samples. At the LCSB, we provide access to these techniques by customized and commercial pipelines and develop targeted bioinformatics analysis methods to integrate the high-dimensional data.

ADVANTAGES:

  1. Inexpensive capturing of whole cell transcriptome on a single cell level (Drop-seq).
  • Possibility to link scRNA-seq data to 11 or more barcoded protein antibodies (CITE-seq)
  1. 10x Genomics can offer even higher transcriptome capturing ratio, which makes it a great tool for investigating nuclei or cells with low transcript abundance (snRNAseq or ATAC-seq)
  • With in-house developed analyses pipeline together with some of the leading software available ICS can offer expertise and support dealing with this type of high dimensional data.
  • We have applied scRNA-seq methods in wide range of project from cancer metastasis, immunological responses up to neurodegeneration.

Technology Owner

University of Luxembourg

Research & Technology Organization

Sigmar Lampe

Counsel IP and Licensing / European Patent and Trademark Attorney at University of Luxembourg

Related keywords

  • Biological Sciences
  • Gene - DNA Therapy
  • Biology / Biotechnology
  • Cellular and Molecular Biology Technology
  • E-Health
  • Health information management
  • Applications for Health
  • Medicine, Human Health
  • Medical Research
  • Genome Research
  • Applications software
  • Genetic Engineering / Molecular Biology
  • Recombinant DNA
  • Other recombinant DNA
  • Medical Health related
  • genomics

About University of Luxembourg

Research & Technology Organization from Luxembourg

The University of Luxembourg, founded in 2003, is a European research university with a distinctly international, multilingual and interdisciplinary character. It is a modern institution with a personal atmosphere, close to European institutions, innovative companies and the financial place.

With nearly 6,200 students and about 1,700 employees from all over the globe, the University offers a unique mix of international excellence and national relevance, delivering knowledge for society and businesses. The University has three faculties and three interdisciplinary centers:

• The Faculty of Science, Technology and Communication (FSTC)
• The Faculty of Law, Economics and Finance (FDEF)
• The Faculty of Language and Literature, Humanities, Arts and Education (FLSHASE)
• The Interdisciplinary Centre for Security, Reliability and Trust (SnT)
• The Luxembourg Centre for Systems Biomedicine (LCSB)
• Luxembourg Centre for Contemporary and Digital History (C²DH)

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