Technology Offers Page 84

RAMOT at Tel Aviv University Ltd. posted this:

Fibrosis is a pathological condition characterized by excessive collagen deposition and leading to loss of function of affected organs. Nearly 45% of all deaths in the developed world are attributed to some type of chronic fibrosis yet a potent antifibrotic drug is still not in sight. Quantitation of fibrosis is important to assess the progression of the disease and evaluate new antifibrotic drugs. Many available methods are invasive and the available non-invasive methods, which generally depend on serum marker, are not fully satisfactory. Therefore, there is a desperate need to develop non-invasive diagnostic methods of fibrosis that can be used alone or in combination with existing methods. Towards this end we developed simple, sensitive, specific and highly reproducible ELISA assays to determine the concentrations of a new marker of collagen biosynthesis, procollagen C-proteinase enhancer-1 protein (PCPE-1), in mouse and human blood. The normal blood concentrations of mouse and human PCPE-1 were determined. Furthermore, using two experimental mouse models of fibrosis (muscle fibrosis in mdx mice lacking dystrophin and liver fibrosis in CCl4 treated mice), we showed that PCPE-1 plasma concentrations increased by up to 50% in both models of fibrosis. In the liver fibrosis model we showed a decrease to normal blood levels upon recovery. In conclusion, our assay nicely reflects tissue fibrosis in mice, providing a new quantitative basis for evaluation of fibrosis. We trust this new non-invasive assay method can be developed into a useful diagnostic tool both for clinical and research applications. It can attract pharmaceutical companies, companies selling diagnostic kits, and companies selling reagents for extracellular matrix research. Project ID : 5-2011-244

RAMOT at Tel Aviv University Ltd. posted this:

Silver exhibit antibacterial activity in either ionic or metallic forms, albeit by two different mechanisms: silver ions penetrating the bacterial cell interact with the respiratory chain and with cellular DNA. Metallic silver – mostly applied as silver nano-crystals – affects primarily cell membrane by physically 'puncturing' it or by slow release of silver ions resulting from oxidative environment. Both forms - silver nano-crystals or silver ions attached to ion exchanging polymers, mostly applied as impregnated dressings, suffer from poor migration from the supplied 'reservoir' to and into their targeted microbial biofilm. While ionic silver has poor migration due to interaction with chloride and other anions, resulting in insoluble salt precipitation, silver nano crystals migration is physically restricted. We have recently developed novel protein-silver hybrids, comprised of a biologically active glucose oxidase core, coated with a thin (~1.5nm) outer layer of metallic silver. The silver-glucose oxidase hybrid retains enzymatic activity and serves as a unique antibacterial agent, generating silver ions in situ. The soluble silver–glucose oxidase hybrid diffuses into the targeted biofilm vicinity and penetrate into its larger pores. Upon scavenging of glucose traces inside the biofilm, the glucose oxidase core of the hybrid produces hydrogen peroxide, capable of locally oxidizing and dissolving metallic silver, thus affecting enzymatically attenuated in situ silver ions release from the hybrid's silver coating into the immediate vicinity of the targeted cells, resulting in effective disinfection. Project ID : 10-2011-255