IVAN RODRIGUEZ ROSELLO posted this:
Licensing Manager at UNIVERSIDAD DE ALICANTE
University of Vigo posted this:Procedure for sea urchin embryo cryopreservation and attached bioassayThis sea urchin (Paracentrotus lividus) embryo cryopreservation procedure with attached bioassay has been specifically designed for sea urchin blastula (8 hour development embryo) conservation in liquid nitrogen to be used as out of reproductive season biological supply. This protocol has applications in research, aquaculture and ecotoxicology. The process described is fully developed, from how to obtain blastula embryos from sea urchins, composition and concentration used of the cryoprotecting agent, cryopreservation procedure including the cryoprotecting agent addition and dilution methodology, the seeding temperature during freezing and including a post-thawing viability study The possibility of a sea urchin embryo biobank ensures not only the access to test organism embryos all year round but also access to these embryos in places where it was not possible before either for their distance to the ocean or for the lack of the necessary facilities to maintain reproductive adults in optimum conditions to breed. The development of the bioassay using cryopreserved sea urchin embryos will help overcome the seasonal constraints in application of bioassays to assess marine water quality.
Universidad de Cádiz posted this:An improved fast and high throughput method for the sequential measurement of nitrate and nitrite in water samplesWe have developed an improved fast and sequential protocol that permits the determination of low concentrations of nitrite and nitrate in aquatic samples using small volumes. It is ideal to analyse large sets of samples with different characteristics, since it is enough a small amount of water the volume and cost of reagents and consumables are reduced.
Maria João Cardoso posted this:
Science and Technology Manager at UATEC - Unidade de Transferência de Tecnologia
Unitat de Valorització de la URV posted this:
Licensing Manager at Fundació URV