CREB-UPC

Automatic system for electroporation of adherent cells growing in standard multi-well plates

Posted by CREB-UPCResponsive · Patents for licensing · Spain

Summary of the technology

- In situ electroporation on standard culture plates.
- Microelectrodes allow the use of low voltages (<20 V) improving safety.
- Low cost manufacturing process for disposable (single use) devices.
- Possibility of manual use or with an automatic positioning system.
- Enhanced and uniform electric field distribution across cell monolayer.
- Possibility of real time monitoring by electrical impedance spectroscopy.
- Non contact between cells and electrodes, reducing the invasiveness of the operation and avoiding causing any physical stress to the biological sample.

Description of the tech for sale

Nowadays, electroporation is a useful technique to introduce foreign impermeable material into the cell cytoplasm. Traditional in vitro equipment performs electroporation in cuvettes where cells are suspended. However, previous trypsinization process is needed when adherent cells are electroporated, causing additional stress to the cells that may affect both the electroporation efficiency and the invasiveness of the operation. In these cases, in situ electroporation is more suitable. Some microfabricated devices have been designed to apply the electric field pulses directly to the adherent cell monolayer. However, most of these devices are custom-built setups, not suitable for their direct use in standard multi-well plates and cells are in contact with the electrodes interfering its normal development. In addition, there are no real time methods for monitoring electroporation efficiency .

Specifications

The present invention solves the above described drawbacks. An electrode assembly has been designed, able to be positioned momentarily on top of cell monolayer keeping a short distance between electrodes and cells. This setup allows to perform non contact in situ electroporation on cells growing on standard multiwell culture plates. Electric field across cell monolayer is enhanced thanks to the electrodes design. This electrode assembly can be a disposable device due to the low cost manufacturing technology (Printed Circuit Board).

Main advantages of its use

  • - In situ electroporation on standard culture plates.- Microelectrodes allow the use of low voltages (<20 V) improving safety.- Low cost manufacturing process for disposable (single use) devices.- Possibility of manual use or with an automatic positioning system.- Enhanced and uniform electric field distribution across cell monolayer.- Possibility of real time monitoring by electrical impedance spectroscopy.- Non contact between cells and electrodes, reducing the invasiveness of the operation and avoiding causing any physical stress to the biological sample.

Applications

  • Transfection tool for research facilities. Pharmaceutical industries. Hybridoma Production – to manufacture antibodies.

Additional information (attached documents)

Additional information (attached documents)

Related keywords

  • Biological Sciences
  • Electronics, IT and Telecomms
  • Speech Processing/Technology
  • Signal Processing
  • System and transportation
  • Fuel cells
  • Other Processes
  • Stem cell Technologies
  • Food Processing
  • Standards
  • Genetic Engineering / Molecular Biology
  • Electronics Related Market
  • Medical Health related
  • Other system software
  • Hydro-electric
  • Electric companies
  • electroporation
  • automatic system
  • adherent
  • cells
  • multi-well
  • plates

About CREB-UPC

Technology Transfer Office from Spain

The Biomedical Engineering Research Centre (CREB) of Universitat Politècnica de Catalunya (UPC) is a multidisciplinary research center in biomedical engineering with over three decades of experience. Our aim is to support the needs of research, technological development, innovation and knowledge transfeer companies, hospitals and institutions related to the field of medical technologly. Our professionals, more than 80 researchers are doctors (50%) and graduates in engineering and other disciplines. Our specialized laboratories enable us to offer solutions for any need or demand from the biomedical field, and as a result, the CREB-UPC acts as an activation tool in the science, technolgoy and business. The CREB-UPC brand TECNIO part of the Government of Catalonia which brings together key actors and experts in applied research and technology transfer in catalonia. It should also be noted that CREB has quality management programs and support programs for the management of innovation in the business sector. The CREB is able to solve scientific and technological problems, acting as a center of support for technology transfer and training to the industry and the field of Helath Science. In the early 90's given the idea of coordinating efforts and maximize resources for a corporate image by different research groups working in the field of Biomedical Engineering at the UPC.

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