Summary of the technology
Regarding the cationic systems developed, the principal innovative aspects are the following:
- They are non viral nanoscopic synthetic molecules able to internalise nuclear material inside different cellular lines. This turns them into good candidates to be considered as non viral vectors in gene therapy processes.
- They are non toxic in a concentration range adapted for the accomplishment of different biomedical studies.
- They present antibacterial properties against gram + and gram- bacteria
- They are able of interacting, in a electrostatic way, with anionic drugs which turns them into drug delivery systems.
- They present a hydrophobic carbosilane skeleton that allows the molecules to cross the cellular membranes with major facility.
- They are soluble in water.
Regarding the anionic systems developed, the principal innovative aspects are the following:
- They have an important antiviral capacity
- They are able to inhibit the infection for HIV
- They have antiinflammatory capacity
- They are good candidates for the production of a microbicide gel of topic use that prevents the infection of the virus across sexual transmission.
Description of the technology
BIOINDEN research group, from the Inorganic Chemistry Department of Alcalá University has develop a technology consisting in the synthesis and structural characterization of dendritic systems of nanoscopic size with applications in different fields of biomedicine. Among all of them the group launches the development of these systems for the transport and release of drugs as a vehicle of transfection in gene therapy or as therapeutic agents (antibacterial, antiviral or antiprionics). The group is looking for companies from the chemistry, pharmaceutical or biotechnology sector for collaboration in R&D agreements, joint venture agreements and licensing agreements.
The developed technology lies in the preparation of molecules of nanoscopic size based on dendritic systems or hyperbranched polymers that contains a carbosilane skeleton and are functionalized in the periphery with groups from different nature (cationic, anionic or neutral), with the objective to provide them with activity focused in different biomedical aims. Among these applications, we can emphasize the development of cationic dendrimers appropriate for the transport of anionic drugs or as non viral vehicles in transfection processes in gene therapy, because of its capacity to join biomolecules with negative charge as oligonucleotides or siRNA. These cationic systems can also act as therapeutic antibacterial agents (against gram + bacterial and gram – bacterial) or as anti-prionic agents. The group has proved that cationic dendritic systems of carbosilane type are able to join by an electrostatic interaction oligonucleotides or small RNA of interference (si RNA), making complexes that have proved not to be toxic in concentration among 1-5 m. These complexes are able to internalise the genetic material inside different types of cellular lines and in some cases they have showed a great efficiency in the inhibition of HIV. They have also showed to be useful in processes of transfection of plasmids to different cellular carcinogenic lines in vitro and in vivo, being observed in some cases percentages of transfection bigger to those that are obtained by the use of commercial transfectants. Regarding the antibacterial capacity of these cationic systems, the group has proved that they present a great activity against gram + bacteria and gram – bacteria, and in addition there are signs of strengthening of the antibacterial activity of penicillin when it joins one of the dendritic systems. The technology developed allows also the preparation of anionic systems that present an important antiviral activity, mainly against HIV. That’s why, these system are proposed as good candidates for the development of a powerful microbicide for external use that avoids the infection for HIV by sexual transmission. Besides this microbicide capacity, these systems have showed antiinflammatory properties what reinforces his inhibitory capacity. The functionalization with neutral groups, has been carried out incorporating oligosacharides into the periphery of the dendritic systems developed what awards to these systems antimicrobial properties. All the synthesized macromolecules have been characterized using different techniques of structural elucidation as elementary analysis, spectroscopy of RMN, IR or UV, mass spectrometry or GPC.
Main advantages of its use
- Regarding the cationic systems developed, the principal competitive advantages are the following:- Cheaper preparation than other commercial transfection agents.- Minor toxicity than other commercial transfection agents..- Levels of transfection similar or superior to commercial alternatives (depending on the cellular line used)- Antibacterial capacity against to a wide spectrum of bacteriaRegarding the anionic systems developed, the principal competitive advantages are the following:- They present a great antiviral capacity, mainly against HIV virus.- They have antiinflammatory activity.- They can be in use in the development of a microbicide gel of topic use.
- Companies from the chemistry, pharma or biotechnology sector.