UATEC - Unidade de Transferência de Tecnologia

Separation of nucleic acids using chromatographic matrices functionalized with ionic liquids

Posted by Maria João from UATEC - Unidade de Transferência de TecnologiaResponsive · Patents for licensing · Portugal

Summary of the technology

Novel multimodal chromatographic supports prepared by functionalizing matrices with ionic liquids, allowing the separation of RNA and DNA in a single chromatographic step with high selectivity, robustness and reproducibility. In comparison to other commercially available supports, a higher dynamic binding capacity for RNA was obtained.

UATEC - Unidade de Transferência de Tecnologia

CONTEXT

Nucleic acids are an important target in research, with a growing importance as therapeutics in the (bio)phar­maceutical industry. Independently of the final applica­tion, it is necessary to separate the nucleic acids from contaminants, which is usually achieved using chroma­tographic strategies that may be expensive and laborious, and often lead to low purity and dena­tured RNA samples.

The innovative nature of the developed novel chroma­tographic supports rely on the application of ionic liquids as multimodal ligands immobilized in a matrix for the separation of RNA and DNA, allowing to obtain high purity and high quality. These supports display high ro­bustness, reproducibility, and selectivity, and may be regenerated. Globally, this strategy is an excellent and improved alternative to obtain RNA samples for distinct applications.

BENEFITS

HIGH SELECTIVITT in the separation of RNA and DNA, which allows to obtain RNA with:

  • HIGH PURITY LEVELS
  • LOWER SALT CONCENTRATIONS
  • LOWER PERIOD IN THE CHROMATOGRAPHIC SYSTEM
  • LOWER DEGRADATION

HIGH REBUSTNESS

HIGH REPRODUCIBILITY

REGENERATION FEASIBILITY with no significant changes in their separation performance.

APPLICATIONS

The prepared chromatographic supports allow the separation/purification of:

NUCLEIC ACIDS from bacterial lysates

The supports may also be prepared for the separation and purification of other specific biomolecules, or from distinct sources .

DEVELOPMENT STAGE

TRL 4: small scale prototype built in a laboratory envi­ronment.

With additional support, it is reasonable to assume that 6 months will be sufficient to scale-up and further com­mercialization.

The experimental protocol to produce this technology is implemented at a lab scale, and the inventors are available to present the technology.

KEYWORDS

Nucleic acids

Ionic liquid

Chromatography

Purification

DEVELOPED BY

Researchers from Aveiro Institute of Materials (CICECO) from the University of Aveiro and from Health Sciences Research Center (CICS-UBI) from the University of Beira Interior.

BUSINESS OPPORTUNITY

Licensing agreement.

PARTNERSHIP

The University of Aveiro seeks industrial partners with interest in the development and/or commercialization of chromatographic supports, as well as partners within the pharmaceutical industry experienced in the produc­tion and/or commercialization of biopharmaceuticals.

Technology #CI18002

Intellectual property status

Patent already applied for
110626
Portugal

Technology Owner

UATEC - Unidade de Transferência de Tecnologia

Technology Transfer Office

Related keywords

  • Physical Sciences and Exact Sciences
  • Chemistry
  • Separation Technologies
  • Biological Sciences
  • Gene - DNA Therapy
  • Biology / Biotechnology
  • Biochemistry / Biophysics Technology
  • Cellular and Molecular Biology Technology
  • Genetic Engineering Technology
  • Synthetic Biology
  • Ionic Liquids
  • Therapeutic
  • chromatography
  • nucleic acids

About UATEC - Unidade de Transferência de Tecnologia

Technology Transfer Office from Portugal

The University of Aveiro is a young and innovative university, which fosters entrepreneurship and technology and knowledge transfer, with expertise in a wide range of knowledge areas namely IT, Life Sciences (Biology, Chemistry, Biochemistry, Health, Environmental Sciences), Nanotechnology and Materials Science, Mechanics, Physics, Mathematics, Building, Design and Management. UATEC is the Technology Transfer Office of University of Aveiro. This office engages in value adding activities such as start-up and spin-off creation, support to university-industry cooperation (sponsored research and consultancy services), IP protection and prosecution, licensing, marketing, among others. It covers four main interrelated functional areas – Intellectual Property Management, Licensing, Entrepreneurship, and Industry Liaison.

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