Summary of the technology
E. coli mazEF-mediated cell death is a quorum sensing process that involves a signal peptide molecule that termed the Extracellular Death Factor (EDF). Unlike the previously discovered quorum sensing factors of E. coli, homoserine lactones and AI-2, EDF is a peptide. EDF is an integral part of the E. coli mazEF-mediated cell death program.
Project ID : 12-2006-612
Description of the technology
The invention brings a new modality of anti-bacterial treatment as a stand-alone therapy or combined with antibioticsThe bacterial toxin MazF induces bacterial cell death by triggering downstream death pathways. The labile anti-toxin MazE is constantly synthesized by bacteria and inactivates MazF by sequestering it.
- Several antibiotics, such as rifampicin, chloramphenicol, mitomycin C act by affecting the MazF/MazE balance.
a Peptide-Based Induction of Bacterial Cell Death :
- The invention is based on the recently discovered Extracellular Death Factor (EDF). EDF is a secreted bacterial pentapeptide which induces MazF-mediated cell death. EDF is the only naturally-occurring peptide known to act as a ‘quorum sensing' DEATH factor.
- EDF target bacteria includes different E.coli strains. Staphylicoccus aureus, Psedomonas aeruginosa, and Bacillus subtilis EDF-like factors are under current research.
- The innovation concerns also EDF antagonists, which may confer antibiotic sensitivity by inducing mazEF plasmid loss.
- EDF-based antibacterial composition may be used for sterilizing as well as for pharmaceutical purposes.
- Possibility of modifying the EDF original sequence by the addition of amino acids and other moieties, thus improving physiological properties and allowing different administration modes.
The Applications for use:
Current Development Stage
- The list of conditions induced by potentially EDF-targeted bacteria includes: diarrhea, dysentery, hemorrhagic colitis, pyogenic infection, abscesses, septicaemia, pneumonia, meningitis, lung infection, cystic fibrosis.
- EDF-based compounds provide a new and specific venue to induce bacterial cell death.
Proof of concept established in different E.coli strains.