Summary of the technology
Coated and functionalized magnetic nanoparticles for e.g. detection and separation of DNA and RNA, protein purification, cell separation and immunoassays, con-trolled by temperature. Contrary to state-of-art mag-netic nanoparticles presently used for separation, which always present a certain degree of aggregation, the present technology proposes nanoparticles with tem-perature-controlled magnetic response.
The magnetic response of these new nanoparticles is suppressed at higher temperatures (tunable between 40°C and 100°C) avoiding aggregation and is enhanced at lower temperatures promoting separation.
Description of the technology
Magnetic particles, nanoparticles and beads are widely used for the capture, separation and detection of analytes. Such magnetic nanoparticles need to have two conflicting characteristics: the need to be highly magnetic for an effective separation and sensing and they need to be lowly magnetic during the capture stage for an effective interaction with the analyte, i.e., no agglomeration should occur during capture and fast agglomeration should occur during separation. Current approach explored in commercial nanoparticles is based on a compromise: magnetic response is not too high nor too low.
This technology bases on nanoparticles with a high magnetic response bellow ≈40°C and a low magnetic response above ≈40°C, such that the capture stage can be performed above 40°C and agglomeration is then induced by lowering temperature.
The present magnetic nanoparticles can be used on several biotechnological processes and assays, such as:
DNA and RNA detection and separation
On-off magnetic response
Temperature-controlled magnetic response:
- Controlled particle aggregation
- Enhanced capture of analytes of interest: particularly effective in applications in which temperature increase is already a mandatory step (e.g. PCR)
- Controlled on-off magnetic signal: reduced errors and false positives in magnetic detections
Similar surface reactivity to current nanoparticles (magnetite), with allows the application of current functionalization approaches
International patent application filled (PCT/IB2018/053697; priority date: 27/05/2017).
TRL 3: The concept has been demonstrated in laboratory.
Standard coatings and functionalization have been tested, with success. In other words, currently strategies and processes used for detection and separation can be applied to our magnetic cores.
Joint further development
The University of Aveiro seeks manufacturers of nanoparticles and/or kits for magnetic detection and separation wanting to enhance their portfolio with innovative, unique and differentiating solutions.
Technology Transfer Office