Consejo Superior de Investigaciones Científicas

Chimeric protein with high antimicrobial activity

Posted by Consejo Superior de Investigaciones CientíficasResponsive · Patents for licensing · Spain

Summary of the technology

Researchers from CSIC have developed a chimeric protein (ClyRODI-H5) with an improved lytic
activity against Staphylococcus sp. and higher stability than the parental endolysins of the state of the
art. Thus, the invention relates to this chimeric protein and its use as an antimicrobial for treating
and/or preventing staphylococcal infections without generation of resistant bacteria; for removing
and/or preventing staphylococcal biofilms formation on abiotic surfaces, or for removing and/or
preventing staphylococcal contamination of foods. Thus, the present invention belongs to the field of
the treatment of bacterial contamination and/or infections.
Industrial partners or companies from the food or pharmaceutical field are being sought to collaborate
through a patent license agreement.

An offer for Patent Licensing

Consejo Superior de Investigaciones Científicas
Consejo Superior de Investigaciones Científicas

ClyRODI-H5: A protein to kill Staphylococcus aureus


Control ClyRODI-H5 Staphylococcal removal after 5 min of treatment with 0.1 µM of ClyRODI-H5 The rapid rise and dissemination of multi-drug resistant (MDR) pathogens represents an urgent global risk for human health, sustainable food production and development. S. aureus is one of the major bacterial agents causing foodborne diseases in humans and many types of infections.
Moreover, its ability to form biofilms onto different surfaces makes this bacterium difficult to eradicate. The study of bacteriophage-encoded lytic proteins (endolysins), able to kill the bacteria through peptidoglycan degradation followed by osmotic lysis, has attracted great interest in the last years. Phage lytic proteins have even been proposed as disinfectants for the food industry. The rapid bactericidal action and biodegradability of phage enzyme-based antimicrobials are appealing features. In this regard, here we have constructed a new chimeric protein (ClyRODI-H5) that comprises a catalytic domain (CHAP) from the endolysin LysRODI and a cell wall binding domain (SH3) from endolysin LysH5.

Main innovations and advantages


· The chimeric lytic protein ClyRODI-H5 shows increased activitycompared to the parental endolysins: 3 and 24-fold higher thanLysRODI and LysH5 respectively. It is active against S. aureus includingmethicillin-resistant S. aureus, and also against other staphylococcalspecies.
· A low concentration (0.1 µM) of CLyRODI-H5 lyses the staphylococcalpopulations in a very short period of time, achieving a completebacterial removal (106 CFU/ml) in 5 min. Besides, the addition of sub-lethal doses of the protein does not trigger staphylococcal resistance atin vitro conditions.
· ClyRODI-H5 is stable in a broader range of pH from 3 to 11, keepingits activity unaltered.
· The addition of ClyRODI-H5 (0.7 µM) strongly prevented (98%) thebiofilm formation onto polystyrene. Moreover, the treatment of pre-formed biofilms with 2.5 µM of the protein was able to remove the

Intellectual property status

Granted Patent
-
European priority patent application

Granted Patent
-
European priority patent application

Granted Patent
-
European priority patent application

Technology Owner

Consejo Superior de Investigaciones Científicas

Public Agency

Related keywords

  • Agrofood Industry
  • Technologies for the food industry
  • Food quality and safety
  • Micro- and Nanotechnology related to agrofood
  • Medical Health related
  • Agro and Marine biotech
  • Food and feed ingredients
  • Food and Beverages

About Consejo Superior de Investigaciones Científicas

Public Agency from Spain

The Spanish National Research Council (in Spanish 'Consejo Superior de Investigaciones Científicas (CSIC)') is a government agency for basic and applied scientific research.

It is the largest public research organization in Spain, with presence in all the Autonomous Communities through 126 centers and 145 associated units.

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