uacoopera

Fast and non-sequential measurement method for the light response of chlorophyll a fluorescence

Posted by uacoopera from uacooperaResponsive · Patents for licensing · Portugal

Summary of the technology

New method for the measurement of the in vivo emission of chlorophyll a fluorescence by photosynthetic organisms, through the generation of light-response curves with independent observations, measured in a non-sequential and much faster way than the existing methods. The method combines the (i) projection in the sample of spatially separated light beams of different intensity or quality, using a digitally-controlled projector, with (ii) the measurement of the emitted fluorescence as a response to each light beam using an image fluorometer.

UATEC - Unidade de Transferência de Tecnologia

CONTEXT

The measurement of the in vivo emission of chlorophyll a fluorescence using a type of instrument called “pulse modulation amplitude fluorometer” is a technique widely used in fundamental and applied studies on the photosynthetic activity of plants, lichens, algae and other photosynthetic organisms. One of the most com­monly used methods consists in the measurement of “light response curves”, based on the quantification of fluorescence emission of a sample exposed to different levels of light intensity.

The methods currently used are affected by two main problems: they are time consuming and based on independent measurements. This technology enables the construction of light response curves consisting of independent measurements in a much faster way than any previously described method.

BENEFITS

NON-SEQUENTIAL MEASUREMENTS

TEMPORALLY-INDEPENDENT MEASUREMENTS

MUCH FASTER than the established methods.

HIGH FLEXIBILITY: to define and control the range of light intensity and color applied to the samples.

APPLICATIONS

Method to be used as a modular component to couple to fluorometry imaging systems, with several applica­tions in photosynthetic organisms:

EVALUATION OF PHYSIOLOGIC STATES

PREDICTION OF VARIATIONS IN PHOTOSYNTHETIC EFFICIENCY UNDER DIFFERENT LIGHTNING CONDITIONS

IDENTIFICATION AND SELECTION OF VARIETIES WITH HIGHER PHOTOSYNTHETIC EFFICIENCY

DEVELOPMENT STAGE

TRL 4: tested in laboratory and available for presenta­tion.

The method was extensively tested in a wide variety of samples and measurement conditions. Protocols and operating parameters were defined enabling to guide the development of new equipment (Serôdio et al. 2013 Plant Physiology).

KEYWORDS

Chlorophyll a fluorescence

Pulse amplitude modulation (PAM) fluorometry

Light curves

Digital projector

Light mask

DEVELOPED BY

Researchers of Centre for Environmental and Marine Studies (CESAM) of the University of Aveiro.

BUSINESS OPPORTUNITY

License agreement.

Joint further development.

Testing of new applications.

Adaptation to specific needs.

PARTNERSHIP

The University of Aveiro seeks partners within the sector of electronic equipment development and manu­facture, as well as other universities and research institutes.

Technology #CI13021

Intellectual property status

Granted Patent
107052
Portugal

Technology Owner

uacoopera

Technology Transfer Office

Additional information (attached documents)

Related keywords

  • Process Plant Engineering
  • Lighting, illumination
  • Physiology
  • Agriculture and Marine Resources
  • Agriculture
  • Sylviculture, Forestry, Forest technology
  • Electronics Related Market
  • Plant health

About uacoopera

Technology Transfer Office from Portugal

The University of Aveiro is a young and innovative university, which fosters entrepreneurship and technology and knowledge transfer, with expertise in a wide range of knowledge areas namely IT, Life Sciences (Biology, Chemistry, Biochemistry, Health, Environmental Sciences), Nanotechnology and Materials Science, Mechanics, Physics, Mathematics, Building, Design and Management. uacoopera is the Technology Transfer Office of University of Aveiro. This office engages in value adding activities such as start-up and spin-off creation, support to university-industry cooperation (sponsored research and consultancy services), IP protection and prosecution, licensing, marketing, among others. It covers four main interrelated functional areas – Intellectual Property Management, Licensing, Entrepreneurship, and Industry Liaison.

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