Summary of the technology
New method for the measurement of the in vivo emission of chlorophyll a fluorescence by photosynthetic organisms, through the generation of light-response curves with independent observations, measured in a non-sequential and much faster way than the existing methods. The method combines the (i) projection in the sample of spatially separated light beams of different intensity or quality, using a digitally-controlled projector, with (ii) the measurement of the emitted fluorescence as a response to each light beam using an image fluorometer.
The measurement of the in vivo emission of chlorophyll a fluorescence using a type of instrument called “pulse modulation amplitude fluorometer” is a technique widely used in fundamental and applied studies on the photosynthetic activity of plants, lichens, algae and other photosynthetic organisms. One of the most commonly used methods consists in the measurement of “light response curves”, based on the quantification of fluorescence emission of a sample exposed to different levels of light intensity.
The methods currently used are affected by two main problems: they are time consuming and based on independent measurements. This technology enables the construction of light response curves consisting of independent measurements in a much faster way than any previously described method.
MUCH FASTER than the established methods.
HIGH FLEXIBILITY: to define and control the range of light intensity and color applied to the samples.
Method to be used as a modular component to couple to fluorometry imaging systems, with several applications in photosynthetic organisms:
EVALUATION OF PHYSIOLOGIC STATES
PREDICTION OF VARIATIONS IN PHOTOSYNTHETIC EFFICIENCY UNDER DIFFERENT LIGHTNING CONDITIONS
IDENTIFICATION AND SELECTION OF VARIETIES WITH HIGHER PHOTOSYNTHETIC EFFICIENCY
TRL 4: tested in laboratory and available for presentation.
The method was extensively tested in a wide variety of samples and measurement conditions. Protocols and operating parameters were defined enabling to guide the development of new equipment (Serôdio et al. 2013 Plant Physiology).
Chlorophyll a fluorescence
Pulse amplitude modulation (PAM) fluorometry
Researchers of Centre for Environmental and Marine Studies (CESAM) of the University of Aveiro.
Joint further development.
Testing of new applications.
Adaptation to specific needs.
The University of Aveiro seeks partners within the sector of electronic equipment development and manufacture, as well as other universities and research institutes.
Intellectual property status
Technology Transfer Office
Additional information (attached documents)