Centre Technology Transfer CITTRU

Improving stem cell culture using carnivorous plants

Posted by Radoslaw from Centre Technology Transfer CITTRUResponsive · Patents for licensing · Poland

Summary of the technology

Mesenchymal Stem Cells (MSCs) are multipotent precursor cells with the unique capacity to self-renew, proliferation and
transformation into specialized cells present in many body tissues, such as muscle, adipose tissue, fibroblasts, cartilage, tendons, and nervous tissue. They are also characterized by the ability to affect the immune system by secretion of compounds
that support the immune response directed among others against bacteria and parasites. They have properties enabling the secretion of chemical compounds that stimulate the formation of new blood vessels. All these attributes make them applicable in regenerative medicine, transplantology and in the treatment of many diseases.

The umbilical cord blood and Wharton’s jelly MSCs are the sources from which MSCs are obtained. Due to the small amount of MSCs in the starting material, it is necessary to lead in vitro cultures to obtain sufficient numbers of cells that can be administered to the patient. Methods of isolation and culture of mesenchymal stem cells, including the growth medium, play a crucial role in obtaining the appropriate biological material that guarantees therapeutic success. Therefore, continuous improvement of procedures for isolating and in vitro culturing of MSCs is extremely important. The subject of the offer is a method of culturing mesenchymal stem cells using secretions from carnivorous plants, which increase cell adherence and proliferation, counteract their transformation into specialized cells, therefore, they longer maintain their pluripotency.

Centre Technology Transfer CITTRU
Centre Technology Transfer CITTRU

Description of the technology

The subject of the offer is a method of culturing mesenchymal stem cells using secretions from carnivorous plants, which increase cell adherence and proliferation, counteract their transformation into specialized cells, therefore,
they longer maintain their pluripotency.

Two types of human stem cells were used for the study:

1. isolated from adipose tissue according to the standard procedure
2. isolated from Wharton’s jelly by explant method

Adherence, apoptosis, and proliferation of mesenchymal stem cells in vitro culture using the plant secretions.

The observed density of cells in the culture using secretions was much higher than in the control culture. In the
control culture, the medium was changed every 3-4 days. In cell cultures, carried out with the plant secretion, the medium exchange was necessary every 2-3 days. This is an indication of the improved proliferation and adherence of cells cultured on
the substrate with plant secretion. Analysis of the level of genes expression associated with the process of apoptosis and proliferation, with regard to endogenous control (the reference gene was the GAPDH gene) showed an increase in three anti-apoptotic genes (BCL-2, NAIP, BIRC2) and a decrease in the expression of one apoptotic gene (BAX).

Analysis of the expression of selected genes encoding membrane proteins that allow the cells to adhere to one another or to the intercellular matrix (matrix) has shown that the contact of stem cells with plant secretions increases the expression of two genes coding for adhesion proteins (AMIGO1 and CLDN1). The beneficial effect on the expression of these genes increases the
ability of the cells to adhesively adhere to the medium, thereby improving their growth and proliferative potential.

The potential of pluripotency of mesenchymal stem cells cultured in vitro using plant secretions

Analysis of the level of expression of genes associated with transcription factors (SOX9, SOX2, NANOG) and genes associated with cell pluripotency (POU5F1 and KLF4), showed that the use of plant secretions according to the developed method increases the pluripotency potential of cells and prevents their differentiation.

Current development status

Laboratory prototypes

Intellectual property status

Patent already applied for

Patent application number : P 426289, P 428366, P 428393

Where : Poland

Desired business relationship

Technology selling

Patent licensing

Technology development

Adaptation of technology to other markets

Intellectual property status

Patent already applied for
P 426289
Poland

Patent already applied for
P 428366
Poland

Patent already applied for
P 428367
Poland

Technology Owner

Centre Technology Transfer CITTRU

Technology Transfer Office

Related keywords

  • Physical Sciences and Exact Sciences
  • Biological Sciences
  • Medicine, Human Health
  • Gene - DNA Therapy
  • Stem cell Technologies
  • Biology / Biotechnology
  • Genetic Engineering Technology
  • In vitro Testing, Trials Technology
  • Enzyme Technology
  • Medical/health
  • Genetic Engineering / Molecular Biology
  • Agricultural genetic engineering applications
  • Medical genetic engineering applications
  • Other Genetic Engineering
  • In vitro Testing, Trials Market
  • Stem cells and biobanks
  • Cellular and Molecular Biology Market
  • Gene Expression, Proteom Research Market
  • Genetic Engineering Market
  • Medical Health related
  • In-vitro diagnostics
  • Therapeutic
  • Agro and Marine biotech
  • Clinical Medicine
  • Plant health
  • Other

About Centre Technology Transfer CITTRU

Technology Transfer Office from Poland

Centre for Technology Transfer CITTRU is a part of Jagiellonian University, whose role is to promote university research, to support innovation and to create cooperation with the business. CITTRU main task is to offer the scientific achievements of the Jagiellonian University in the market by providing legal protection, licensing, sale of intellectual property rights, creation of academic business, coordination of company-ordered research projects, etc. Currently promoted technologies are mainly focused on new materials science, chemistry, pharma and medical technology.

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