Summary of the technology
High throughput high content image screening analysis, using an In Cell Analyzer 2000 (INCA 2000, GE healthcare). The system automatically acquires multiple fluorescence-labeled images from live or fixed cells cultured in multi-titter-well plates and analyzes a wide range of cellular types, with intracellular biological markers, bearing different fluorescent labels. The image data analysis quantifies the multi-parametric information at the cellular and sub-cellular levels allowing graphical visualization and characterization.
Project ID : 12-2011-206
Description of the technology
The Facility has an In Cell Analyzer 2000 (INCA 2000, GE healthcare), the first in Israel, which is a high throughput high content image screening analyzer capable of automatically acquiring multiple fluorescence-labeled images from live or fixed cells cultured in multi titter-well plates. We offer the possibility to analyze a wide range of cellular types together with intracellular biological markers baring different fluorescent labels. The image data analysis is performed in batches, using specialized software that quantifies the multi-parametric information at the cellular and sub-cellular levels allowing graphical visualization, characterization and analysis.
Cell count, cell cycle, cell migration, vesicular labeling, cytotoxicity, DNA repair, cell adhesion, cell proliferation, apoptosis, gene expression reporter systems, neurite outgrowth, etc.
Technichal features of INCA 2000 include:
- Environmental control
- Liquid handling
- Transmitted and fluorescence light imaging
- Temperature Control
- Plates or slide imaging
In addition, the lab is equipped with:
- Cell and tissue culturing facilities
-Human primary cells, human embryonic and adult stem cell cultures and various differentiation protocols.
-Custom assay preparation
- Drug HT screening facility (not yet available)
-Liquid handling robot integrated with INCA 2000
Dr. Miguel Weil
Laboratory for Personalized Medicine and Neurodegenerative Diseases
Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences. Tel Aviv University
Dr. Leonardo Solmesky
Britannia Bldg. Room 238a, Tel: firstname.lastname@example.org