RAMOT at Tel Aviv University Ltd.

High Content Screening Facility

Posted by RAMOT at Tel Aviv University Ltd.Responsive · Innovative Products and Technologies · Israel

Summary of the technology

High throughput high content image screening analysis, using an In Cell Analyzer 2000 (INCA 2000, GE healthcare). The system automatically acquires multiple fluorescence-labeled images from live or fixed cells cultured in multi-titter-well plates and analyzes a wide range of cellular types, with intracellular biological markers, bearing different fluorescent labels. The image data analysis quantifies the multi-parametric information at the cellular and sub-cellular levels allowing graphical visualization and characterization.
Project ID : 12-2011-206

RAMOT at Tel Aviv University Ltd.

Description of the technology

The Facility has an In Cell Analyzer 2000 (INCA 2000, GE healthcare), the first in Israel, which is a high throughput high content image screening analyzer capable of automatically acquiring multiple fluorescence-labeled images from live or fixed cells cultured in multi titter-well plates. We offer the possibility to analyze a wide range of cellular types together with intracellular biological markers baring different fluorescent labels. The image data analysis is performed in batches, using specialized software that quantifies the multi-parametric information at the cellular and sub-cellular levels allowing graphical visualization, characterization and analysis.

Assays:
Cell count, cell cycle, cell migration, vesicular labeling, cytotoxicity, DNA repair, cell adhesion, cell proliferation, apoptosis, gene expression reporter systems, neurite outgrowth, etc.

Technichal features of INCA 2000 include:

  • Environmental control
  • Liquid handling
  • Transmitted and fluorescence light imaging
  • Temperature Control
  • Plates or slide imaging

In addition, the lab is equipped with:

  • Cell and tissue culturing facilities
    -Human primary cells, human embryonic and adult stem cell cultures and various differentiation protocols.
    -Custom assay preparation
  • Drug HT screening facility (not yet available)
    -Compound Libraries
    -Liquid handling robot integrated with INCA 2000

Contact

Dr. Miguel Weil
Laboratory for Personalized Medicine and Neurodegenerative Diseases
Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences. Tel Aviv University

Tel: 03-640-6981miguelw@tauex.tau.ac.il

Facility Manager:

Dr. Leonardo Solmesky
Britannia Bldg. Room 238a, Tel: 03-640-5874
leonardo@post.tau.ac.il

http://www.tau.ac.il/lifesci/departments/cell_r/members/weil/weil.html

Project manager

avi Wener
Research services

Project researchers

Miguel Enrique Weil
T.A.U Tel Aviv University, Life Sciences
Cell Research and Immunology

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About RAMOT at Tel Aviv University Ltd.

Technology Transfer Office from Israel

Ramot is Tel Aviv University's (TAU) technology transfer company and its liaison to industry, bringing promising scientific discoveries made at

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