Interferometric phase microscopy for label-free morphological evaluation of sperm cells
Knowhow and Research output
Technology Offer
Interferometric phase microscopy for label-free morphological evaluation of sperm cells
- RAMOT at Tel Aviv University Ltd.
- From Israel
- Responsive
- Knowhow and Research output
Summary of the technology
Nondestructive quantitative method utilizing DHM for detecting morphology of non-motile favorable organelle for IVF.
350 sperm cells from healthy donored were tested according to WHO criteria and showed that label-free IPM can identify sperm cell abnormalities, with an excellent correlation with label-based bright-field microscopy (BFM), and with higher accuracy compared with label-free BFM
Project ID : 5-2014-805
Details of the Technology Offer
Portable, compact, add-on Interferometric Phase Microscope module
An optical module and software package which optically compresses several interferometric images into a single camera image and thus provides extended interferometric the field of view (FOV).
Records more interferometric information on the same amount of camera pixels.
Applications
Medical industry
In-vitro diagnostics -Measuring quantitative thickness profiles of biological cells without any labeling and nondestructive quality testing.
Electronics industry
Real-Time, Rapid profiling during or after lithography processes, and for efficient inspection of electric circuits during or after fabrication.
Advantages
Quantitative, Subnanometric, Optical Thickness Imaging
- High throughput. Thousands of frames per second in wide field (no scanning)
- Non-contact manner
- Label-free (no sample preparation is needed)
- Minimal level of noise.
- Ambient conditions (good for live samples)
Real-Time, Non-Labeled, Quantitative, 3D Sperm Morphology profile
- Head, Neck and Tail morphology
- Cell Motion and Speed
- Cell Vitality
- Tail Spinning direction
Watch video: ">https://www.youtube.com/watch?v=lBX5f5TptBM