A rapid, colorimetric nucleic-acid detection approach that uses unmodified colloidal gold nanoparticles (AuNPs) and a sequence-specific oligonucleotide “oligotargeter”. After hybridization of the oligotargeter to a target nucleic acid (optionally following amplification), AuNP aggregation behavior changes and produces a visible color shift (e.g., red vs blue), enabling straightforward positive/negative determination and supporting kit-based infectious disease testing workflows.
What it is: A diagnostic method and kit for detecting target nucleic acids using unmodified gold nanoparticles plus at least one oligotargeter that binds a target sequence.
How it works (detection principle): The oligotargeter is allowed to hybridize (anneal) to the target nucleic acid. Gold nanoparticles are then introduced. Free (unbound) oligotargeters can interact with AuNPs and help prevent nanoparticle aggregation; aggregated AuNPs shift color (e.g., red to blue). When target nucleic acid is present, oligotargeters bind the target, reducing free oligotargeter available to prevent aggregation, producing a colorimetric change that indicates presence of the target sequence.
What can be included in the kit: AuNPs + oligotargeter(s), and optionally items for sample handling and nucleic-acid workflows (e.g., preservatives/additives, extraction/purification materials, reaction buffers, positive/negative controls, primers/probes, containers, color chart, IFU). \n\nRepresentative targets described: Kits are described for detecting multiple pathogens/targets including Acinetobacter, mycobacteria (e.g., TB complex), staphylococcus (including MRSA), HBV, HIV, Influenza, and West Nile virus (among others as detailed in the specification).
Current development status
Experimental technologies
Applications
Point-of-care / near-patient infectious disease testing
Rapid pathogen screening in clinical labs
Molecular diagnostics kits where a simple visual readout is desirable
Potential adaptation for field diagnostics (resource-limited settings) depending on assay workflow and validation.
Desired business relationship
Patent licensing
Joint ventures
Technology development
New technology applications
Adaptation of technology to other markets
The American University in Cairo (AUC) is committed to teaching and research of the highest caliber and provides exceptional liberal arts and professional education in a cross-cultural environment. With a diverse campus community and strong academic ecosystem, AUC advances research, applied innovation, and real-world impact in Egypt and beyond. AUC’s Technology Transfer Office supports this mission by protecting university intellectual property and partnering with industry to license technologies and enable spin-outs.
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