This technology is a paper-based analytical platform that replicates the format and functionality of conventional 96-well plates while overcoming bulk-averaged measurement limitations.
The 96-Puddle Paper Plate (96-PPP) enables spatially confined and reproducible reactions, allowing time-resolved and quantitative analysis using standard laboratory equipment or simple optical readouts such as smartphones.
It provides high reproducibility across puddles, compatibility with existing assay workflows, and significantly reduced cost and fabrication complexity.
The platform expands reliable bio/chemical analysis beyond traditional plastic well plates and centralized laboratories.
The 96-Puddle Paper Plate (96-PPP) is a lab-on-paper analytical platform designed to resolve spatially heterogeneous reaction behaviors that are obscured in conventional bulk well-plate assays. Traditional liquid-filled wells collapse diffusion, convection, and localized reaction dynamics into a single averaged signal, often resulting in poor reproducibility, misleading interpretation, and limited quantitative reliability.
The proposed platform uses silk-screened PDMS barriers on a cellulose substrate to form uniform, leakage-free micro-puddles arranged in a standard 96-well format. This structure preserves spatial reaction significance while remaining fully compatible with existing microplate readers, fluorescence microscopes, and colorimetric imaging systems. Quantitative agreement with conventional 96-well plates has been experimentally validated, while achieving significantly lower signal variation across individual puddles.
By enabling structured reaction confinement, the platform supports time-resolved observation of condition-dependent reaction dynamics, including enzymatic reactions, aggregation processes, redox reactions, and metabolite sensing. Its paper-based, disposable, and storage-efficient design allows low-cost manufacturing, easy scalability, and application-specific customization.
The technology is well suited for consumable supply businesses, assay developers, diagnostic and research tool companies, and collaborative R&D partners seeking scalable, reproducible, and spatially resolved analytical platforms beyond traditional plastic well plates.
IBU Inc. is a technology commercialization and consulting firm supporting licensing, co-development, and venture creation based on deep-tech and research-originated technologies.
The company operates as a technology transfer partner, startup studio, and accelerator, working with universities, research institutes, and industry players.
IBU Inc. connects technology owners with corporate partners and investors by structuring collaboration models, commercialization strategies, and market-oriented pathways.
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