Dynamic patterns of DNA modification are part of epigenetic regulation in vertebrates, including humans, which contributes to normal phenotypic variation and disease risk and thus can be used as diagnostic and prognostic disease markers. We offer a robust and economical nucleotide-resolution technique (TOP-seq) for high-resolution mapping of epigenetic modifications in the genome using covalent tagging of the modification target sites.
Determination of genomic DNA modification profiles for research and diagnostics.
FIELDS OF APPLICATION
Epigenomic profiling, biomarkers, genomics, diagnostics, cell-free DNA.
Patents: EP2776575 (B1), US9347093 (B2), US9988673 (B2).
Applicant: Vilnius University.
• Saulius KLIMAŠAUSKAS
• Edita KRIUKIENĖ
• Zdislav STAŠEVSKIJ
Kriukienė et al. (2013)Nature Commun., 4: 2190.
Staševskij et al. (2017)Mol.Cell, 2017, 65: 554.
Daniūnaitė et al. (2019)Curr.Opin.Biotechnol., 2019, 55: 23
Current development status