Leading Biology

Insect Baculovirus Expression Vector System

Posted by Leading from Leading BiologyResponsive · Research Services and Capabilities · United States

Summary of the technology

Advantages of insect baculovirus expression system

1). Safe
2). Large capacity of carrying foreign genes
3). High expression level of exogenous protein
4). The expressed eukaryotic gene products can be properly folded and modified, thus have good bioactivity.

Description of the technology

Insect baculovirus expression vector system introduction

Insect baculovirus expression vector system (BEVS) is a system in which baculovirus worked as a vector of foreign genes, by infect insect cells or insect larvae to produce exogenous proteins. It is an important part of the eukaryotic expression system.

Advantages of insect baculovirus expression system

1). Safe

2). Large capacity of carrying foreign genes

3). High expression level of exogenous protein

4). The expressed eukaryotic gene products can be properly folded and modified, thus have good bioactivity.

Advantages

Compared to mammalian cell expression system:

1). The culture conditions are relatively simple and do not require the CO2

2). The cost is relatively reasonable

3). Not easy contaminated by bacteria

4). High protein content

The technical advantages of Leading Biology

1. We use classic Bac-to-Bac technology to create a fast, simple and efficient baculovirus expression platform. The technique can produce recombinant virus quickly, and do not require tedious plaque assay, which greatly improves the efficiency.

2. The production cycle is short and the flux is large. Can achieve the process of both small scale cell culture and large scale fermentation, the protein products can even reach the level of 50mg.

3. Provide free virus ion amplification and storage services, and the shelf-life can as long as half a year.

Service process

Classic case

The expression of Rsignal1 recombinant protein in Sf9 cells.

After codon optimization, the Rsignal1 target gene is constructed on the insect expression vector, then transfected with Sf9 cells to get P1 baculovirus. Through a series of virus amplification and optimal MOI exploration and high yield affinity purification, finally obtained the high quality Rsignal1 protein.

Fig1.P1 expression test

Fig.2 The optimal MOI test

Fig. 3 Purification test

Fig. 4 Final product

Introduction of insect baculovirus expression vector

Common expression vectors and their features

Vector

Features

pFastBac1

Strong AcMNPV polyhedron(PH) promoter for high level protein expression

Large multiple cloning site for simplified cloning

pFastBacHT

Strong polyhedron(PH) promoter for high-level protein expression

N-terminal 6His tag for purification of recombinant fusion protein using metal-chelating resin and aTEV protease cleavage site for removal of the 6His tag following protein purification

Vector supplied in 3 reading frames for simplified cloning

pFastVacDual

Two strong baculovirus promoters(PH and p10) to allow simultaneous expression of two proteins

Two large multiple cloning sites for simplified cloning

Common expression cells and their features

Cell

Sorce

Application

Sf9

The Sf9 cell lineis a clonal isolatederived from IPLBSF21-AE (Sf21), and are originated from the ovary tissue of Spodoptera frugiperda chrysalis.

Suitable for transfection, purification, production of high titer virus, cell plating and recombinant protein expression.

Sf21

Sf21 cellis also a clonal isolatederived from the IPLBSF-21 cell line and also are originated from the ovary tissue of the pupae of the Spodoptera frugiperda.

Also suitable for transfection, purification, production of high titer virus, cell plating and recombinant protein expression. Sometimes, Sf21 cells may have a higher protein expression rate than Sf9 cells

Hi5

Hi5 cell lines discovered by Boyce Thompson Institute during the study of plants, they are originated from cabbage looper ovary cells and Trichoplusiani cells

Hi5 cells could express high quality recombinant virus. It is also widely used for transfection and plaque purification, moreover, the Hi5 cell line is more suitable for the expression of secretory recombinant protein.

S2

S2 are late fruit fly embryo cells. Most of them are female tetraploid cells, and there are also some diploid cells.

It is suitable for the expression of recombinant protein

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Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

  • Innovative configurations of chromatography columns custom tailored to each protein
  • Vigorous quality control system to ensure the required quality and reproducibility
  • Production capacity of up to tens of grams
  • Flexible scale-up protein production
  • Competitive price with fast turnaround time

Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email usinfo@leadingbiology.com

Order Products:Order Related Products

By Phone: 1-661-524(LBI)-026d2 (USA)

Technology Owner

Leading Biology

Small and Medium Enterprise

Leading Biology

Business Development Manager at Leading Biology

Related keywords

  • Biological Sciences
  • Biology / Biotechnology
  • Protein Engineering
  • biology
  • protein expression

About Leading Biology

Small and Medium Enterprise from United States

Reliable biological products supplier in Richmond, CA. With modern product development and commercialization timelines, always accelerating your productivity. We offer more than 30,000 self-developed antibodies & proteins, hundreds of ELISA kits, and proteins. Our research focuses on 4 key areas: Antibody Discovery, Protein Expression, Phase Display & Antibody Engineering. https://leadingbiology.com/article-946.html

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