Polyclonal Antibody Serum Preparation

Introduction

Leading Biology provides fully customizable services for polyclonal antibody production ranging from peptide design and synthesis to antibody purification and detection. A variety of different hosts to produce polyclonal antibodies are used, including rabbit, rat, mouse, goat, guinea pig, chicken, sheep, monkey, etc. Each project will be discussed thoroughly before starting the protocol so as to sure that your demands will be fulfilled. 

Polyclonal antibodies (pAbs) are a group of antibodies that could bind to different epitopes of the same antigen. Even if a virus or bacteria changes its structure just a little bit, the antibodies will still be able to find and attach to it, which is different from the single combination of monoclonal antibodies.

That is why researchers often use polyclonal antibodies to identify parts of unknown molecules. Apart from that, the polyclonal antibody preparation is cheaper, quicker and easier in comparison to monoclonal antibody preparation.

Service process and package

Each custom service will be paid unique attention with details so that you can achieve the next biotechnology breakthrough. Also, you will receive updates on project status so you can better know about the process.

Classic Case

1

Publication：Nature（letters）

IF：41.456

Title：A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defense

Name：anti-FLS2 antibody

Type：Rabbit polyclonal antibody    

Species: Arabidopsis thaliana

2

Publication：Cancer Science     

IF：3.054

Title：Phloroglucinol suppresses the metastatic ability of breast cancer cells by inhibition of epithelial-mesenchymal cell transition       

Name：FN1; mouse polyclonal antibody

Type：Mouse polyclonal antibody

Species: human Fibronectin

3

Publication：Tumor and Stem Cell Biology     

IF：5.013

Title：MicroRNAs Regulate Tumor Angiogenesis Modulated by Endothelial Progenitor Cells Name：Rat anti-mouse miR-10b/miR-196b

Type：rat monoclonal antibody

Species: mouse

4

Publication：Plos One

IF：3.05

Title：Phosphodiesterase Type 4 Inhibitor Rolipram Improves Survival of Spiral Ganglion Neurons In Vitro      

Name：Anti-200kD Neurofilament antibody 

Species: mouse   

5

Type：chicken polyclonal antibody 

Publication：FEBS letters             

IF：3.169

Title：Multiple FtsZ2 isoforms involved in chloroplast division and biogenesis are developmentally associated with thylakoid membranes in Arabidopsis

Name：anti-FtsZ2 antibody

Type：Rabbit polyclonal antibody

Species: Arabidopsis thaliana    

Service details
 

Antigen Forms
Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.

Antigen Quantity
We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.

Antigen Concentration
Leading Biology can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.

Considerations for Gel Bands
Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands, in particular, it is important to maintain a higher concentration of the protein as outlined above.

Shipping Antigens to Leading Biology

Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS, or DHL Overnight to the address listed on our Order Form. 

Related Services

Chicken Polyclonal Antibody Service

Mouse Polyclonal Antibody Service

Please obtain a quote before ordering, and refer to the quote number when you place an order.
Have a Question? Email us info@leadingbiology.com
By Phone: 1-661-524(LBI)-0262 (USA)

so that you can achieve the next biotechnology breakthrough. Also, you will receive updates on project status so you can better know about the process.