Leading Biology

Protein Expression in Bacillus Subtilis

Posted by Leading from Leading BiologyResponsive · Research Services and Capabilities · United States

Summary of the technology

Advantages of the Bacillus subtilis expression service in Leading Biology.
1. Having a variety of Bacillus subtilis expressing vectors, such as pHT101 and pHT254.

2. Having many different expressions hosts, such as intracellular expression host AS1, 1012, 168, secretory expression host WB800N, etc to fulfill different requirements of customers.

3. Could prepare high efficient competent Bacillus subtilis.

Description of the technology

Bacillus subtilis expression system introduction

1. Simple cell wall composition, the secreted protein products don’t contain endotoxin.

2. The ability to produce a large number of extracellular/target proteins, simple recovery and purification.

3. Having a high efficient secretory signal peptide and molecular chaperone system, could achieve high efficient secretory of target protein, avoid the formation of inclusion bodies.

 

Advantages of the Bacillus subtilis expression service in Leading Biology

1. Having a variety of Bacillus subtilis expressing vectors, such as pHT101 and pHT254.

2. Having many different expressions hosts, such as intracellular expression host AS1, 1012, 168, secretory expression host WB800N, etc to fulfill different requirements of customers.

3. Could prepare high efficient competent Bacillus subtilis.

 

Service process

 

Classic case

An influenza virus capsid protein, molecular weight 100kD, the customer provides genetic information, after genetic optimization, the vector was constructed and expressed in Bacillus subtilis 168 and 1012:

                                                                        

Left: Expression of target protein in 168   Right: Expression of target protein in 1012

A/B indicate different expression vectors-negative control

 

 

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Whole cell-based protein expression system

 

Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.

Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.

Working with us, you will get the guaranteed service to accommodate your requirements.

 

  • Innovative configurations of chromatography columns custom-tailored to each protein
  • Vigorous quality control system to ensure the required quality and reproducibility
  • Production capacity of up to tens of grams
  • Flexible scale-up protein production
  • Competitive price with fast turnaround time

 

Contact Information

 

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

Order Products: Order Related Products

By Phone: 1-661-524(LBI)-0262 (USA)

Technology Owner

Leading Biology

Small and Medium Enterprise

Leading Biology

Business Development Manager at Leading Biology

Related keywords

  • Biological Sciences
  • Biology / Biotechnology
  • Protein Engineering
  • protein expression
  • life science

About Leading Biology

Small and Medium Enterprise from United States

Reliable biological products supplier in Richmond, CA. With modern product development and commercialization timelines, always accelerating your productivity. We offer more than 30,000 self-developed antibodies & proteins, hundreds of ELISA kits, and proteins. Our research focuses on 4 key areas: Antibody Discovery, Protein Expression, Phase Display & Antibody Engineering. https://leadingbiology.com/article-946.html

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