Candidate proteins for mutant p53 conversion into wild type conformation, found in embryonic stem cells
Knowhow and Research output
Technology Offer
Candidate proteins for mutant p53 conversion into wild type conformation, found in embryonic stem cells
- RAMOT at Tel Aviv University Ltd.
- From Israel
- Responsive
- Knowhow and Research output
Summary of the technology
The tumor suppressor, p53, is mutated in over 50% of human tumors, disabling its ability to bind DNA and induce transcription of its target genes. The aim is to stabilize mutant p53 into wild type conformation and restore its tumor suppressive activity. We found that mutant p53 conformation is stabilized towards a wild-type conformation in mouse embryonic stem cells. We proceeded and performed mass spectrometry and found several candidate proteins for the stabilization of the mutant form into a wild type form. It might be possible to use the knowledge we obtained to produce peptides or increase protein expression of such proteins (by chemical inducers etc.) in malignant cells harboring mutant p53 and thus convert mutant p53 into wild type conformation, thus restoring its tumor suppressive activity leading to apoptosis of the malignant cells. We hope that by knocking down these factors in ESCs we can show that their presence is leading to the conversion of mutant p53 into wild type conformation and that in their absence mutant remains in its mutant conformation.
Project ID : 10-2013-696
Details of the Technology Offer
The invention provides methods for stabilization of mutant p53 in embryonic stem cells by interacting proteins, compositions comprising the same and uses thereof for treatment of various conditions associated with mut-p53, such as, cancer.
Additional information can be provided upon request.