A range of cell delivery systems are crafted for intracellular delivery such as cationic lipids, protein transduction agents or cell-penetrating peptides. Despite of the large number of techniques, transport of macromolecules are rare and only a small portion of these systems are capable of transporting proteins and only very few of them are delivering antibodies. Possible ways for macromolecular translocation is through endocytosis. In some existing applications there are cell-penetrating peptides with a residue number of about 20 amino acids and these molecules are triggering the clathrin-dependent endocytosis. In the recent patent a short WYKYW pentapeptide is applied to deliver relatively large cargo (antibodies, for example) inside the cell via lipid-raft/caveolin-dependent manner through binding to ganglioside GM1.
The WYKYW peptide based delivery system - in contrast with earlier peptide based delivery systems - targeting the GM1 ganglioside and initiate lipid-raft/caveolin-dependent endocytosis of the peptide and the delivered cargo molecule. Targeting the lipid-raft/caveolin-dependent endocytosis has advantages up against the clathrin-dependent endocytosis. One of these is that the progression of internalized caveolae to endosomes and later lysosomes is very slow or absent, so that allow time for the delivered cargo to escape to the target organelles or cytosol. This way of internalization is also desired as direct escape from internalized compartments of the cargo is much more rapid. Our results showed that WYKYW-tagged IgG complex were successfully delivered into live mammalian cells at extracellular concentrations ranging between 20-160 nM and the escape of the protein was observed. We have found that the internalized compartment is not fused with lysosomes and the cargo molecule left the compartment in an intact state.
Desired business relationship
Patent licensing
Current development status
Laboratory prototypes
Top of the Hungarian University education; more than 21000 students, with about 4100 students from foreign countries; the highest level of reseach in Hungary; intellectual property portfolio of 41 patent, 8 know-how; numerous parts of the IP prtfolio is licenced or expoited in spin-off enterprises;
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