Anti importin beta1 monoclonal antibody

  • Yeda
  • From Israel
  • Responsive
  • Patents for licensing

Summary of the technology

A highly specific and sensitive anti-Importin b1 (KPNB1) monoclonal antibody. The mAb recognizes an epitope
comprising residues 301-320 of human KPBN1 and is highly specific for cytoplasmic KPNB1 in diverse
applications.

Yeda

Description

Importin b1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and
nucleocytoplasmic transport, hence there is keen interest in its characterization and subcellular interactomes.
However, the current efficiency of proximity-dependent biotin identification (BioID) in detecting importin complex
cargos is very limited. Therefore, Prof. Fainzilber and his team generated a highly specific and sensitive antiKPNB1 monoclonal antibody, which enables Biotinylation by Antibody Recognition (BAR) analysis of importin b1
interactomes. The monoclonal antibody recognizes an epitope comprising residues 301-320 of human KPBN1, and
strikingly is highly specific for cytoplasmic KPNB1 in diverse applications, with little or no reaction with KPNB1 in
the nucleus. BAR with this novel antibody revealed numerous new interactors of importin b1, expanding the KPNB1
interactome to cytoplasmic and signaling complexes that highlight potential new functions for the importins complex
beyond nucleocytoplasmic transport. Additional data is available on Biorxiv https://www.biorxiv.org/content/10.1101/2022.03.23.485495v1 [1]

Related Keywords

  • Biological Sciences
  • Biology / Biotechnology
  • Cellular and Molecular Biology Technology
  • Medicine, Human Health
  • Medical Research
  • Industrial Products

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WIS is one of the world’s leading multidisciplinary basic research institutions in the natural and exact sciences. It is located in Rehovot, Israel, just south of Tel Aviv. It was initially established as the Daniel Sieff Institute in 1934, by Israel and Rebecca Sieff of London in memory of their son Daniel. In 1949, it was renamed for Dr. Chaim Weizmann, the first President of the State of Israel and Founder of the Institute.

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