University of Huelva posted this:New procedure for the genetic transformation of plant cells and kit for carrying out said methodThe strategy for transformation is extremely simple and does not involves subcloning the gene of interest in any vector. The kit is not based on specific promoters or regulatory DNA sequences, thus it can be applied to many different plant species, from unicellular microalgae to higher plant protoplasts. Since the strategy is not based on specific promoter or regulatory DNA sequences, only the marker and the gene of interest sequences are inserted in the microalgal genome, avoiding the introduction of undeserible DNA sequences in the transformant strain. This is very important given the concern that transfer of exogenous DNA through transgenesis causes in the public opinion
Benemérita Universidad Autónoma de Puebla posted this:BACTERIAL FORMULATION FOR PLANT GROWTH.The present invention includes a bacterial formulation that contains 6 different bacterial strains: Azospirillum brasilense Sp7, Burkholderia unamae MTI641, Pseudomonas putida KT2440, Sphingomonas sp. OF 178, Gluconoacetobacter diazotrophicus PAI 5 and Bradyrhizobium sp. MS22, able to promote the growth in plants of agricultural and/or bioremediation interest. The formulation promotes the growth of maize plants and other plants of agricultural interest. The bacteria of the present invention are adhered and may colonize the plants in a suitable manner by means of their own capacities.
UATEC - Unidade de Transferência de Tecnologia posted this:Production of a novel collagenase and applicationsThe described process for collagenase production and purification is rapid, cheap and reproducible. The obtained product is highly active and stable for long period storage (in solution or lyophilised). The features of this novel product are, certainly related to a novel domain organization and architecture not yet described for other collagenases
Dr.Shivraj Dasari posted this:
CEO at SLS cell cure technologies Pvt. Ltd
Benemérita Universidad Autónoma de Puebla posted this:Bacterial formulation by plant growthThe present invention includes a bacterial formulation that contains 6 different bacterial strains: Azospirillum brasilense Sp7, Burkholderia unamae MTI641, Pseudomonas putida KT2440, Sphingomonas sp. OF 178, Gluconoacetobacter diazotrophicus PAI 5 and Bradyrhizobium sp. MS22, able to promote the growth in plants of agricultural and/or bioremediation interest. The formulation promotes the growth of maize plants and other plants of agricultural interest. The bacteria of the present invention are adhered and may colonize the plants in a suitable manner by means of their own capacities.
Cracow University of Technology posted this:Suspension containing gold or silver nanoparticles or a mixture thereof and a production method thereofThe present invention relates to a process for the preparation of nanosilver and nanogold using an aqueous extract of dog rose, white grape and knotweed. Substances which were extracted from these plants act both as reducer of silver and gold ions and stabilizing agent of forming silver and gold nanodispersion, preventing them from agglomeration and inhibiting their growth, so that the resulting particle size does not exceed 100 nm.
Cracow University of Technology posted this:Laboratory for Trace Organic Analyses - Cracow Univerisity of TechnologyLaboratory for Trace Organic Analyses is an integral part of the Department of Chemical Engineering and Technology at Krakow University of Technology. The Laboratory is accredited from Polish Centre for Accreditation that signed EA Multilateral Agreement. PCA has become a signatory of EA MLA
University of Vigo posted this:Method for the sequential immunochemical evaluation on a same biological sample of NCAM (Neural Cell Adhesion Molecule) protein and its PolySialic Acid (PSA) tail, by western blot coupled to chemiluminiscence detection.The method optimizes the expression assays of PSA-NCAM isoforms in biological samples available in small quantities or restricted (as is the case of human specimens, for example). It improves quantitative and qualitative performance of PSA-NCAM analysis by western blot.
University of Vigo posted this:Valorization of whey and vinasses by producing antimicrobial extracts using biotechnological processesThis invention is based on the production of antimicrobial extracts with potential application against pathogenic microorganisms. The biotechnological production of antimicrobial metabolites was carried out using Lactobacillus plantarum strains. Two industrial wastes, whey, and wine distilled lees (or vinasses) were assayed in order to make the process economically competitive with regard to the chemical one. The extracts are mainly composed by lactic acid, 3-phenyllactic and bacteriocins. Whey was enzymatically hydrolyzed and the solutions were fermented sequentially or simultaneously. The bioprocesses were carried out continuously or discontinuously
Research & Technology Organization
Universidad de Alcalá-OTRI posted this:Double port injector to corneal transplant DMEKThe Reseacrh group in Cell biology of solid tumors and tisular regeneration from Alcala University and the Research group in Cellular Engineering from University from Hospital La Paz, have developed a new injector designed to reduce endotellial damage during the corneal endothelial graft implantation in eye´s anterior chamber. The group is looking for cooperation or commercial agreement with companies manufacturers of sanitary products and medical devices, ophthalmological clinics and hospitals.
RAMOT at Tel Aviv University Ltd. posted this:A novel behavioral bioassay for preclinical testing and characterization of CNS agentsOur team, including zoologists, statisticians, and computer scientists have developed a new hi-res bioassay for preclinical testing and characterization of CNS agents, extracting key animal-centered parameters of behavior via computerized tracking and measurement of free exploration. The features that distinguish our system and method from other existing systems and methods are: elaborate data preparation for analysis and a methodology that secures replicability of results across laboratories, and exposes active management of kinematic variables. Our measured parameters are carefully designed, animal-centered building blocks of behavior. They are selected on the basis of an in-depth understanding of the mouse’s functional world. These quite unexpected and unusual parameters reflect the animal’s cognitive and emotional state and its information processing capacity; therefore they are most prone to be affected by drugs that act on respective CNS structures that mediate these states. Unlike all other existing systems we expose and quantify the rich and highly organized developmental dynamics of self regulated behavioral growth. The dynamics of this process before and after genetic manipulation and/or pharmacological treatment can now be readily measured by our system, articulating the effects of potential CNS drugs on behavioral growth and decay processes. Project ID : 2-2009-11
RAMOT at Tel Aviv University Ltd. posted this:Oral Mucosa-derived Stem Cells: A Unique Population for the Prevention and Treatment of Ischemic Heart FailureStem cells (SC) are considered crucial building blocks for any regenerative strategy. Adult oral mucosa (OM) is a unique source for generating adult pluripotent stem cell (OPSC) populations. This uniqueness consists in the fact that 95% of the OM whole populations in culture (1st – 8th passages) express markers of embryonic and mesenchymal SC. Thus, in contradistinction to known sources which comprise very low numbers of SC, OPSC generation for clinical use is simple and cost effective and does not require cumbersome cost-consuming purification steps. The general objective is to lay the foundation for the development of a variety of products based on the pluripotency and simple and cost effective generation of billions of OPSC. The major aim of the proposed project is to develop a product for the treatment of ischemic heart failure (IHF). This product which will serve as a proof of concept was selected based on medicals needs, market cap and time to market. The specific aims of the proposal are: 1. To develop a cardiac product aimed at treating ischemic heart failure (IHF). The aim is to test the safety and therapeutic effect of OPSC on experimental IHF in a minipig model. 2. To strengthen the intellectual property by fully characterizing the profile of OPSC. The prevalence of IHF continuously increases (1 million new cases every year in the western world) and currently the market cap is estimated in the range of billions. Project ID : 10-2011-260
RAMOT at Tel Aviv University Ltd. posted this:Immunotherapy for Stroke and Cognition in a Cerebral Amyloid Angiopathy Animal ModelCerebral amyloid angiopathy (CAA) is due to amyloid accumulation in the vessel walls leading to hemorrhagic stroke, and cognitive impairment. There are no available treatments to specifically reduce the risk of CAA. In this research we aim to assess brain tissue damage and cognitive impairment resulting from CAA in animal model and to investigate a novel approach to immune therapy. Methods: We have shown that nasal vaccination with a proteosome adjuvant (Protollin) that is well tolerated in humans, decreases amyloid plaques in an Alzheimer’s disease mouse model. It was recently reported that an overexpression of TGF-?1 under the control of an astrocyte promoter GFAP in mice results in CAA. TGF-?1 mice were nasally treated with Protollin on a weekly basis starting at the age of 13 months for three months. Following treatment animals were subjected for MRI and cognition analysis. Results: Here we show that nasal Protollin activates perivascular macrophage and potently decreases vascular amyloid in TGF-?1 mice. Using MRI we found that while PBS treated animals showed a significant enlargement of the lateral ventricles area, Protollin prevents further brain damage and prevents pathological changes in the blood-brain barrier. Vascular risk factors have been found to be associated with vascular dementia. Using an object recognition test and Y-maze, we found significant improvement in cognition with the Protollin treated group. Interpretation :Our study demonstrates that activation of macrophages by Protollin is a novel approach to reduce microhemorrhage, prevent stroke and improve cognition in a model of cerebral amyloid angiopathy. Project ID : 10-2011-259
Research & Technology Organization
UNIVERSIDAD DE ALICANTE posted this:Novel and simple procedure for dispersing graphene derivatives in chemically curable resins.The Laboratory of Adhesion and Adhesives of the University of Alicante and the company Dental Global Training, have developed a new procedure to incorporate efficiently graphene derivatives (nanofibers, nanosheets, nanoparticles) in chemically cured resins by using an in situ polymerization technique. The method is very simple and fast, it provides an excellent dispersion of the graphene derivatives in both the solid components of the resin and in the cured polymer matrix, and is entirely friendly with the environment. The novel method does not require the use of organic solvents nor water, ultrasounds, and high pressure/temperature. The resulting resin composites show excellent mechanical properties, good wear resistance, easy manipulation and increase the insolubility in oral fluids, and therefor they have special interest in applications in odontology, traumatology and non-medical applications too (automobile, construction, civil engineering, aeronautics, space engineering, electronics and optics). It is looking for companies interested in acquiring this technology for its commercial exploitation.
Barcelona Skin Genomic posted this:Dermatological pharmaceutical composition for the treatment of skin inflammatory diseases, such as dermatitis and atopic dermatitis (Psoriasis, Acne and Itchy Skin)Dermatological formulation, composed of pharmaceutical compounds, antioxidant molecules and additional active ingredients are developed by Barcelona Skin Genomic to treat inflammatory skin diseases with high efficacy during treatment of atopic dermatitis (AD) with high Scoring Atopic Dermatitis (SCORAD) index, Psoriasis, Acne and Itchy Skin.