Yissum - Research Development Company of the Hebrew University

A Novel Biosensor for Detecting Wine Spoilage

Posted by Yissum - Research Development Company of the Hebrew UniversityResponsive · Innovative Products and Technologies · Israel

Summary of the technology

In the proposed research we describe a novel, efficient and low-cost approaches for detection of acetic acid in wines. The first technique is based on the interaction of acetic acid volatiles with a specific receptor from the bacterium Bacillus subtilis. The receptor, which is encoded within a bacterial biosensor, will activate a reporter gene in the presence of volatiles of acetic acid.
Project ID : 8-2018-4662

Yissum - Research Development Company of the Hebrew University

Description of the technology


  • Measurements of volatile acidity in wines can be a challenging issue in many young and aging wines. The primary acid that contributes to volatile acidity in wines is acetic acid. Its presence, above a specific threshold, indicates spoilage of the product. It is therefore important for wine producers and collectors to monitor constantly this compound, particularly during barrel aging which is prone to oxidation.
  • The common method used to monitor volatile acidity in wineries is titration using a Cash Still, a method requiring training as well as a liquid sample of the wine. Furthermore, the high number of barrels in the winery cellar limits the ability of the winemaker to monitor each and every one of the barrels, thus putting the wine at high risk of spoilage in the most oxidation-sensitive stage of the wine making process.
  • Several attempts have been made to produce "electronic noses" that could sense acetic acid in the wine’s headspace, however these devices have a problem coping with the high concentration of ethanol found in wines (which masks their sensitivity to acetic acid) and thus—to our knowledge— have not been successful to date.
  • The proposed research project aims to use a “biological-nose” instead of an “electronic nose.” This biosensing cell is a genetically modified microbial organism, which is insensitive to ethanol, and can efficiently sense and report the presence of acetic acid.

Our Innovation

Microbial biosensors for the quantitative detection of acetic acid in wine’s headspace, thus avoiding the need to obtain a liquid sample or open the wine barrels/bottles altogether.


The prototype will comprise a thin needle that can be introduced into the headspace through the stopper of a wine barrel or a bottle cork, and facilitate inspection of the wine without affecting the liquid or damaging the stopper.

The Opportunity

The proposed technology will lead to a novel, easy-to-use tool for rapid and real-time analysis of wine condition, without the need to obtain a liquid sample, facilitating the rapid evaluation of numerous barrels of wine.

Additionally, the device could be used to detect acetic acid in other food-related products.

The novel sensor enables fast performance of a large number of tests, including each and every barrel and many wine bottles in the cellar, without the need to open the barrels or the bottles. This will significantly improve winemaking practice and assure high quality.

Project manager

Ilya Pittel

Project researchers

Oded Shoseyov
HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences
Plant Sciences and Genetics

Yael Helman
HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences
Plant Pathology and Microbiology

Related keywords

  • Technologies for the food industry
  • Food quality and safety
  • Micro- and Nanotechnology related to agrofood
  • Environment
  • Clean Production / Green Technologies
  • Waste Management
  • Food and Beverages
  • Pollution and Recycling Related
  • Food & Nutrition
  • Food Analysis & Security

About Yissum - Research Development Company of the Hebrew University

Technology Transfer Office from Israel

Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Founded in 1964 to protect and commercialize the Hebrew University’s intellectual property. Ranked among the top technology transfer companies, Yissum has registered over 8,900 patents covering 2,500 inventions; has licensed out 800 technologies and has spun-off 90 companies. Products that are based on Hebrew University technologies and were commercialized by Yissum generate today over $2 Billion in annual sales.

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