Yissum - Research Development Company of the Hebrew University

An Efficient PCR Protocol for co-Amplification of Multiple Markers

Posted by Yissum - Research Development Company of the Hebrew UniversityResponsive · Innovative Products and Technologies · Israel

Summary of the technology

• A new protocol that allows to efficiently co-amplify a large number of loci in the same PCR reaction (multiplex PCR), to generate material that is ready for sequencing without further manipulations
• A two-step procedure, simultaneously amplifying over 30 different and independent markers, hence substantially increasing the sensitivity and specificity of the information acquired
• Successfully used on bisulfite-treated, small volume plasma samples

Project ID : 6-2018-5690

Description of the technology

Keywords

Multiplex PCR, PCR, cfDNA, Research tool, Liquid Biopsy, DNA methylation, cell-free DNA

Current development stage

TRL5 Technology validated in relevant environement

Application

PCR on bisulfite-treated DNA, followed by next generation sequencing, has a major potential as a diagnostic tool, by identifying DNA derived from specific tissue based on cell type-specific methylation markers. To maximize assay sensitivity, multiple loci have to be amplified in parallel. However, multiplex PCR of bisulfite-converted DNA remains a major challenge.

Our Innovation

A new protocol that allows to efficiently co-amplify a large number of loci after bisulfite conversion (multiplex PCR), to generate material that is ready for sequencing without further manipulation.

  • simultaneously amplifying over 30 different and independent methylation markers
  • Increased specificity and sensitivity: detects low levels of strongly diluted differentially methylated loci
  • Compatibility with smaller amount of available bisulfite-converted template
  • Reduced costs and time of procedure

Opportunity

  • Most important tool for simultaneous detection of a large number of markers of the same tissue/state, increasing sensitivity and specificity of the test while reducing overall reagent and labor costs
  • Method validated in multiple studies, including reactions using genomic DNA and second bisulfite-converted cfDNA - the most challenging template
  • Allows for routine, affordable non-invasive detection of tissue specific cell death in the human body

Project manager

Mel Larrosa
VP Business Development Healthcare

Project researchers

Yuval Dor
HUJI, School of Medicine - IMRIC
Developmental Biology and Cancer Research

Related keywords

  • Medicine, Human Health
  • Biology / Biotechnology
  • Genome Research
  • Micro- and Nanotechnology related to Biological sciences
  • Recombinant DNA
  • Monoclonal Antibodies and Hybridomas
  • Gene Splicing and Manufacturing Equipment
  • Other Genetic Engineering
  • Molecular design Market
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  • Micro- and Nanotechnology related to Biological sciences
  • Biochemistry / Biophysics Market
  • Toxicology Market
  • In vitro Testing, Trials Market
  • Stem cells and biobanks
  • Cellular and Molecular Biology Market
  • Population genetics Market
  • Gene Expression, Proteom Research Market
  • Bioinformatics Market
  • Enzymology/Protein Engineering/Fermentation
  • Genetic Engineering Market
  • Diagnostic
  • Therapeutic
  • Other Medical/Health Related
  • Agro and Marine biotech
  • Anatomy, Pathology, Immunology, Physiology
  • Clinical Medicine
  • Life Science & Biotechnology
  • Research / Development tools

About Yissum - Research Development Company of the Hebrew University

Technology Transfer Office from Israel

Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Founded in 1964 to protect and commercialize the Hebrew University’s intellectual property. Ranked among the top technology transfer companies, Yissum has registered over 8,900 patents covering 2,500 inventions; has licensed out 800 technologies and has spun-off 90 companies. Products that are based on Hebrew University technologies and were commercialized by Yissum generate today over $2 Billion in annual sales.

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