Summary of the technology
A new method for culturing contact-dependent cells in suspension
A unique cell line of chicken embryonic fibroblasts (CEFs) that can reach extremely high cell densities in suspensions, was developed using this method
Project ID : 6-2018-4673
Description of the technology
Chicken embryonic fibroblasts (CEFs) are commonly used for the production of vaccines and are a source material for cultured meat. Fibroblasts are contact-dependent limiting their large-scale production to carrier beads capable of reaching only 5-6 million cells/ml.
We developed a method that allows the culture of chicken embryonic fibroblasts (CEF’s) in suspension. We produced a sub-clone of the commonly used DF-1 line which can uniquely reach extremely high cells densities in suspension cultures. The cell line is a continuous line of chicken embryo. The cells are free of endogenous sequences related to avian sarcoma and leukosis viruses and have normal fibroblastic morphology. The cells have higher proliferation rate and also can be sub-cultured infinite number of times.The expected increase in yield is up to 40-fold in comparison to current growing methods
Applications for use
Chicken Embryonic Fibroblasts (CEFs) are commonly used in the industry, and as so, methods for improving large-scale industrial cell production are of need, specifically for vaccine production
The proposed technology can be used by pharmaceutical and biotechnology companies to develop human and veterinary viral and therapeutic vaccines.