Summary of the technology
protein sequence from Hoefleo Phototropica has an extremely high affinity towards biotin. Fragment of this protein was identified to have the sufficient feature of the biotin binding properties.
Project ID : 6-2017-4514
Description of the technology
A unique protein sequence from Hoefleo Phototropica with extremely high affinity towards biotin. The fragment of this protein was identified to have the sufficient feature of the biotin binding properties.
- Increased affinity of binding to biotin
- Increased stability of the biotin conjugate over avidin-biotin
- Increased signal amplification over avidin-biotin
The improved properties of octameric-hoefavidin-biotin might be exploited as an improvementover streptavidin, for applications including ELISA, Blotting, Immunology, Peptide & proteinpurification, DNA labeling & purification, Electron microscopy, Crosslinking, Affinity targeting, Light& fluorescent microscopy, Localization, Drug delivery, Pathology, Affinity therapy, Cell separation,Phage and yeast display technologies, Flow cytometry, Bio-sensing, and Nanoparticle probes.