Summary of the technology
The LCSB Bioimaging Platform (bip) provides access to and support for a broad palette of advanced microscopy and flow cytometry instruments, enabling researchers to perform studies from the tissue level and down to sub-cellular scales.
Description of the technology
Focus of the bip is to grant researchers access to state-of-the-art analytical imaging technologies. As such, the bip comprises advanced microscopy systems for high resolution studies of fixed and live samples as well as for low-phototoxicity imaging of whole organs and organisms. Image analysis is supported by strong in-house capacities and the university’s high-performance computing facility. Platform users are trained to become proficient on individual systems. Access to the platform under collaborative service agreements is possible on demand.
Our team works in close collaboration with platform users and research groups to support instrument use, quality control, data acquisition, analysis, and interpretation. Specific expertise in image analysis enables us to assist users with the development of custom-made software tools.
Training and access to instruments:
- Super-resolution Leica SP8 STED 3X, Confocal Zeiss LSM 710, Spinning Disk Zeiss Cell Observer, High Content Perkin Elmer Opera, Automated epi-fluorescence Nikon Eclipse Ti-Eand Olympus IX83 with multiple light sources
- Selective Plane Illumination Microscope constructed in-house with four laser lines (405, 488, 561, and 633 nm), three objectives (20x water, 25x water with ultra-long working distance, 40x water), and high-precision 4D stage
- Becton Dickinson FACSAriaIII cell sorter, Becton Dickinson LSRFortessa cell analyser
- Training on advanced microscopy and flow cytometry systems
- Support for fixed sample, tissue, and live-cell imaging
- Image analysis and software development
We support users with their data analysis and custom software development needs.
University of Luxembourg
Research & Technology Organization