Creative Animodel

CAR-T Preclinical in vivo Study

Posted by Creative AnimodelResponsive · Research Services and Capabilities · United States

Summary of the technology

Creative Animodel provides customized preclinical testing services for your CAR-T cells. In cooperation with our CAR-T cells production division, Creative Biolabs, we are able to provide you with one-stop full service of both CAR-T production and preclinical testing. We also offer services for customized CAR-T cell toxicity management assessments.

Description of the technology

Engineered Chimeric Antigen Receptor T (CAR-T) cell is the leading immune-therapy in oncology. Clinical trials have produced encouraging results in CAR-T involved cancer treatments. Despite of the positive results from the clinics, CAR-T therapy associated safety issues still pose risks in its clinical applications. Preclinical assessment of CAR-T efficacy and safety are necessary for its dosage determination and risk managements.

Creative Animodel provides customized preclinical testing services for your CAR-T cells. In cooperation with our CAR-T cells production division, Creative Biolabs, we are able to provide you with one-stop full service of both CAR-T production and preclinical testing. We also offer services for customized CAR-T cell toxicity management assessments.

We conduct CAR-T cell preclinical studies in human tumor sample derived xenograft mouse/rat models. Immunodeficient mouse/rat that lack functional B cells, T cells, or NK cells, and with reduced innate immunity are the optimal animal models for engraftment of human tumor cells. In addition, these immunodeficient small animals also support engraftments of modified human immune system, including CAR-T cells, for better clinical modeling.

CAR-T preclinical studies in human tumor xenograft mouse/rat model

Our preclinical study services includes: 
✓ Establishing of xenograft mouse/rat model 
✓ Cytotoxicity assay to determine Effector: Target ratio
✓ CAR-T biodistribution and serum persistence
✓ Evaluation of CAR-T efficacy 
✓ Assessment of CAR-T associated toxicity
✓ Customized toxicity management

General flow scheme of CAR-T preclinical study services
1. Establish tumor xenograft mouse/rat models based on client's purpose of study and assessment of successful engraftments
2. CAR-T administration, sample collection and measurements for tumor targeting efficacy and CAR-T induced toxicity. Technology used includes, but not limited to, flow cytometry analysis, immunohistochemistry, cytokine panel analysis, etc.
3. Animal necropsy for end-point evaluation of CAR-T cell therapy efficacy

Services for CAR-T Pharmacokinetic studies:
✓ CAR-T serum persistence and engraftment
✓ CAR-T bidistribution and tissue infiltration

Services for CAR-T efficacy studies:
✓ Tumor cell measurements: 
    o Size measurements for solid tumor, body weight
    o Tumor cell counts and flow cytometry analysis of peripheral blood and bone marrow for hematopoietic tumor
✓ End point animal necropsy for tissue weighing
✓ Tumor size monitoring by bioluminescent imaging

Services for CAR-T toxicity and safety evaluation:
✓ Cytokine panel analysis (end point or time course)
✓ Blood cell and bone marrow content monitoring by flow cytometry
✓ Cytotoxicity analysis for determine effector: target ratio and cell necrotic risk assessments

Our experienced scientists will help you to develop the study proposal that best suit your purpose and fast advance your drug through clinical trials.

Technology Owner

Creative Animodel

Small and Medium Enterprise

Related keywords

  • Biological Sciences
  • Clinical Research, Trials
  • Biology / Biotechnology
  • Genetic Engineering Technology
  • Medicine, Human Health
  • Genetic Engineering / Molecular Biology

About Creative Animodel

Small and Medium Enterprise from United States

Creative Animodel totally understands that it is a challenging and long-time process to develop novel therapies especially anticancer regimens. As an experienced bioscience CRO, we would like to help you accelerate the process through our preclinical services including PD, PK, toxicology services and the establishment of tumor-bearing animal models, the supply of tumor cell lines and tissues, and the support of tumor related biotechniques. Our highly experienced preclinical team has performed a huge number of PD, PK, and toxicology experiments to evaluate novel compounds. According to our detailed protocol, specific aspects of drug property and its mechanism of action are fully understood. During researches, the study results will be sent to clients in a timely and cost-effective manner. If necessary, clients can also work with our PD, PK or toxicology team to design the most appropriate protocol and monitor the whole experimental process.

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