Yissum - Research Development Company of the Hebrew University

Highly Sensitive Method for DNA Detection of Viral and Bacterial Infections

Posted by Yissum - Research Development Company of the Hebrew UniversityResponsive · Innovative Products and Technologies · Israel

Summary of the technology

In the present study, we implement the isothermal rolling circle amplification (RCA) process to trigger a dendritic RCAinduced formation of amplifying DNAzyme catalytic labels. We describe the dendritic RCA-mediated formation of the Mg2+- dependent DNAzyme or of the hemin/G-quadruplex HRPmimicking DNAzyme units and the use of fluorescence, color, or chemiluminescence as readout signals for the different sensing platforms. Finally, by applying two different circular DNA templates, the multiplexed analysis of two different genes is demonstrated with the parallel detection of the Tay-Sachs genetic disorder mutant and the gene associated with the TP53 pathogen.
Project ID : 6-2014-2993

Description of the technology

The technology enables an affordable and easily deployable method for detection of water/soil contamination, and/or rapid detection of viruses in humans and animals.

The method employs the extension of the isothermal rolling circle amplification (RCA) process to a RCA-stimulated dendritic synthesis of catalytic nucleic acids (DNAzymes) sensing platform.

The method is based on the improvement of the RCA sensing methods by two elements:

  • The introduction of a functional hairpin that regenerates the analyte and allows the dendritic branching of the RCA chains.
  • The isothermal autonomous formation of DNAzymes in the RCA chains.

These catalytic labels enable simple detection of the chemical constituents of interest. By the tailoring of different circular DNA templates that lead to the formation of different RCA-branched DNAzyme reporter catalysts, the multiplexed analysis of different analytes is demonstrated.

Project manager

Mel Larrosa
VP Business Development Healthcare

Project researchers

Itamar Willner
HUJI, Faculty of Science
The Institute of Chemistry

Related keywords

  • Medicine, Human Health
  • Biology / Biotechnology
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About Yissum - Research Development Company of the Hebrew University

Technology Transfer Office from Israel

Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Founded in 1964 to protect and commercialize the Hebrew University’s intellectual property. Ranked among the top technology transfer companies, Yissum has registered over 8,900 patents covering 2,500 inventions; has licensed out 800 technologies and has spun-off 90 companies. Products that are based on Hebrew University technologies and were commercialized by Yissum generate today over $2 Billion in annual sales.

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