Summary of the technology
Novel Approach to Improve Production of Secondary Metabolites in Yeast
Project ID : 8-2012-2767
Description of the technology
Metabolic engineering harnesses subcellular compartments in bakers’ yeast
Food & Nutrition, Functional Foods & Ingredients, Cosmetics
Proof of concept and prototype
Patent application filed in the United States
- Secondary metabolites, such as terpenoids (or isoprenoids), produced by plants, are used in pharmaceuticals, perfumes, coloring agents, and food additives among others.
- The levels of these compounds in plants are low and extraction is costly, inefficient and requires large amounts of plant material, while synthesis involves multiple steps and produces low yields.
- One approach to obtain rapid and inexpensive high level production of plant terpenoids involves metabolic engineering of heterologous organisms that are easily cultivated and extracted.
- Bakers’ or brewers’ yeast, Saccharomyces cerevisiae, provides an attractive platform for this process.
Producing high yields of valuable natural plant compounds in the common yeast, Saccharomyces cerevisiae, by using different subcellular compartments.A yeast line producing high yield of terpenoids has been already developed.
- Up to 20-fold improvement in production of plant derived sesquiterpenoids in yeast.
- The secondary metabolites include alkaloids, terpenoids, tannins, glycosides (steroids and phenolics) and saponins. These have applications in pharmaceuticals, food flavorings, and perfumery.
- Can be used for the production of different secondary metabolites according to specific requirements.
- Seeking industrial cooperation
- May be used to produce natural insecticides, coloring agents, flavors and fragrances, anticancer and antimalarial drugs among others
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