Summary of the technology
Staphylococci (Staphylococcus) are one of the most important human bacterial pathogens. Many methods have been developed for the identification and typing of bacterial isolates of the genus Staphylococcus (Staphylococcus aureus) in recent years. These methods can be divided into two groups: phenotypic and genotypic methods. The results obtained using commercial tests, which are based on biochemical and immunological reactions profiles, are affected by mistakes due to variable expression of phenotypic features and the ambiguity from the interpreting the test results. The overall accuracy of conventional tests is low and it is in the range 50-70%. Among genotypic methods, DNA-DNA hybridisation and 16S rRNA gene sequence analysis have defined the species in the genus Staphylococcus and they are recommended to confirm the results of the new methods that are introduced into general use. However, there is still no universal method for staphylococcal species identification and typing at the same time, which would be widely used in all laboratories and allow to obtain high reproducibility of the results. The proposed method may determine the species identification and phylogenetic relationships in the genus Staphylococcus. It shows a high sensitivity and specificity. The results of this method can be interpret very easy, even in the case of the intraspecific polymorphisms. There is the possibility to differentiate between even very closely related species such as S. delphini, S. intermedius and S. pseudintermedius.
Description of the technology
The subject of this offer is a novel method for species identification and determination of phylogenetic relationships in the genus Staphylococcus. The proposed solution, based on the unique diversity pattern of the selected gene and its sequence polymorphism analysis, facilitates a quick and reliable species identification and determination of phylogenetic relationships.
The subject of the offer is a new use of a genomic hyptf locus in microbiological diagnostics, in particular, to determine the species identification and phylogenetic relationships in the genus Staphylococcus. As an example, the approach can be based on use of PCR (Polymerase Chain Reaction) on the template of conservative genomic locus to obtain a product, digestion of product with restriction enzymes and analysing of the resulting image (RFLP, Restriction Fragments Length Polymorphism) for the species identification. RFLP analysis with appropriately selected restriction enzymes also allows for analysis of intraspecies variability in the genus Staphylococcus.
Main advantages of its use
- a unique pattern of hyptf gene variation
- the ability to easy interpret the test results, even in the case of the intraspecific polymorphisms
- the possibility of obtaining RFLP images of four different restriction enzymes with a unique and reproducible pattern
- the possibility to differentiate between even very closely related species such as S. delphini, S. intermedius and S. pseudintermedius.
- Staphylococci (Staphylococcus) are one of the most important human bacterial pathogens. These bacteria are responsible for many diseases and the colonization of the skin and mucous membranes. Determination of taxonomic diversity in the genus Staphylococcus is complex, and there are currently more than 38 species, based on the sequencing of the gene encoding 16S rRNA. The proposed method has been developed for the identification and typing of bacterial isolates of the genus Staphylococcus (Staphylococcus aureus) and it finds an application in biomedical field.
About Centre Technology Transfer CITTRU
Technology Transfer Office from PolandCentre Technology Transfer CITTRU
Centre for Innovation, Technology Transfer and University Development (CITTRU) is a part of Jagiellonian University, whose role is to promote university research, to support innovation and to create cooperation with the business. CITTRU main task is to offer the scientific achievements of the Jagiellonian University in the market by providing legal protection, licensing, sale of intellectual property rights, creation of academic business, coordination of company-ordered research projects, etc. Currently promoted technologies are mainly focused on new materials science, pharmacology and medical technology. Inventions offered by Jagiellonian University are promoted and awarded during numerous exhibitions, e.g. 58th International Exhibition of Innovation, Research and New Technologies INNOVA (BRUSSELS 2009), 38th International Exhibition of Invention New Techniques & Products (Geneva 2010) or 24th International exhibition of environmental equipment, technologies and services POLLUTEC (Paris 2009).