Summary of the technology
Viral infections are associated with numerous human and animal diseases. However, due to difficulties in identification of viruses and their high variability, the etiological factors of numerous diseases are unknown, as for example in a Kawasaki’s disease. The respiratory diseases are the examples of difficulties in identification of viruses in clinical material. Lasting many years research on these diseases still lead to the identification of new viruses, e.g. human coronavirus NL63 or HKU1, human bocavirus, SARS virus or EMC coronavius. The inability to their identification is due to high variability of viruses and the imperfect detection system.
Currently used methods for the identification of known viruses include a variety of methods, from the cell culture by testing for presence of antigens, to molecular methods allowing for the amplification of nucleic acids. Molecular methods also include methods to identify new viruses or variants of viruses undetectable by standard methods. The most sensitive method for the identification of viruses is the use of universal primers in a standard amplification of nucleic acids. The methodology uses the presence of solid, conservative sites in the genomes of viruses belonging to a single family. The use of such sites for primer design brings a hope that in the case of unknown viruses, these fragments will also be present and it will be possible the amplification and identification of new viruses and new variants of known viruses. The biggest disadvantage of this method is its effectiveness limited to viruses that have the same elements with a length of 18-25 nucleotides, which indicates their relationship. Some convenience in this case is CODEHOP method that allows the use of protein fragments and conservative design of highly degenerative primers. The proposed identification method of RNA viruses finds an application in faster and better diagnosis of the infections of RNA viruses.
Description of the technology
The subject of the offer is a new method for the identification of RNA viruses, which is a potential application to detect known RNA viruses and their variants, as well as for detection of new RNA viruses, which may not be identified by standard methods. The offered method can also be used for the amplification of fragments of RNA, which may find an application in faster and better diagnosis of the infections of RNA viruses.
The invention relates to a new method for the identification of RNA viruses involving the use of short 6-8 nucleotide DNA elements, as the attachment sites of specific primers in PCR reaction in a reverse transcription reaction. Method also includes the synthesis of the second strand of DNA, which allows for the addition to the nucleic acid fragment of the synthetic adapters allowing for subsequent amplification of the genetic material of RNA viruses. This is a modification of a currently applicable standard PCR reaction.
Main advantages of its use
- Ease of implementation.
- Higher specificity compared to currently used methods PCR using universal primers
- The main advantage of this method is a significant reduction of conserved locations neccessary to amplify the nucleic acid as compared to a standard PCR reaction using the universal primers. The resulting products can be analyzed, for example, using size analyses (for example, agarose or polyacrylamide gel) or the use of sequencing. The other advantages of the proposed method are:
- The possibility of applying this method in identification of the viral RNA in clinical samples
- The proposed method allow to detect known RNA viruses and their variants, as well as for detection of new RNA viruses, which may not be identified by standard methods. The offered method can also be used for the amplification of fragments of RNA, which may find an application in faster and better diagnosis of the infections of RNA viruses in the biomedical field.
About Centre Technology Transfer CITTRU
Technology Transfer Office from PolandCentre Technology Transfer CITTRU
Centre for Innovation, Technology Transfer and University Development (CITTRU) is a part of Jagiellonian University, whose role is to promote university research, to support innovation and to create cooperation with the business. CITTRU main task is to offer the scientific achievements of the Jagiellonian University in the market by providing legal protection, licensing, sale of intellectual property rights, creation of academic business, coordination of company-ordered research projects, etc. Currently promoted technologies are mainly focused on new materials science, pharmacology and medical technology. Inventions offered by Jagiellonian University are promoted and awarded during numerous exhibitions, e.g. 58th International Exhibition of Innovation, Research and New Technologies INNOVA (BRUSSELS 2009), 38th International Exhibition of Invention New Techniques & Products (Geneva 2010) or 24th International exhibition of environmental equipment, technologies and services POLLUTEC (Paris 2009).